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Title

Different states of integrin LFA-1 aggregation are controlled through its association with tetraspanin CD9

AuthorsReyes, Raquel ; Monjas, Alicia; Cardeñes, Beatriz; Gilsanz, Álvaro; Machado-Pineda, Yesenia; Cabañas, Carlos
KeywordsCD9
Tetraspanin
Adhesion
Cytotoxicity
LFA-1
Integrin
Issue Date21-May-2015
CitationBiochimica et Biophysica Acta - Molecular Cell Research 1853: 2464- 2480 (2015)
Abstract© 2015 Elsevier B.V. The tetraspanin CD9 has been shown to interact with different members of the β1 and β3 subfamilies of integrins, regulating through these interactions cell adhesion, migration and signaling. Based on confocal microscopy co-localization and on co-immunoprecipitation results, we report here that CD9 associates with the β2 integrin LFA-1 in different types of leukocytes including T, B and monocytic cells. This association is resistant to stringent solubilization conditions which, together with data from chemical crosslinking, in situ Proximity Ligation Assays and pull-down experiments, suggest a primary/direct type of interaction mediated by the Large Extracellular Loop of the tetraspanin. CD9 exerts inhibitory effects on the adhesive function of LFA-1 and on LFA-1-dependent leukocyte cytotoxic activity. The mechanism responsible for this negative regulation exerted by CD9 on LFA-1 adhesion does not involve changes in the affinity state of this integrin but seems to be related to alterations in its state of aggregation.
URIhttp://hdl.handle.net/10261/133553
DOI10.1016/j.bbamcr.2015.05.018
Identifiersdoi: 10.1016/j.bbamcr.2015.05.018
issn: 1879-2596
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