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Title: | Differential action of pateamine A on translation of genomic and subgenomic mRNAs from Sindbis virus |
Authors: | González-Almela, Esther; Sanz, Miguel Ángel CSIC ORCID; García-Moreno, Manuel CSIC; Northcote, Peter; Pelletier, Jerry; Carrasco Llamas, Luis CSIC ORCID | Keywords: | Alphavirus protein synthesis eIF4A Sindbis virus Helicase inhibitor Translation inhibitors |
Issue Date: | 5-Jun-2015 | Publisher: | Elsevier | Citation: | Virology 484: 41- 50 (2015) | Abstract: | © 2015 Elsevier Inc. Pateamine A (Pat A) is a natural marine product that interacts specifically with the translation initiation factor eIF4A leading to the disruption of the eIF4F complex. In the present study, we have examined the activity of Pat A on the translation of Sindbis virus (SINV) mRNAs. Translation of genomic mRNA is strongly suppressed by Pat A, as shown by the reduction of nsP1 or nsP2 synthesis. Notably, protein synthesis directed by subgenomic mRNA is resistant to Pat A inhibition when the compound is added at late times following infection; however, subgenomic mRNA is sensitive to Pat A in transfected cells or in cell free systems, indicating that this viral mRNA exhibits a dual mechanism of translation. A detailed kinetic analysis of Pat A inhibition in SINV-infected cells demonstrates that a switch occurs approximately 4. h after infection, rendering subgenomic mRNA translation more resistant to Pat A inhibition. | URI: | http://hdl.handle.net/10261/133519 | DOI: | 10.1016/j.virol.2015.05.002 | Identifiers: | doi: 10.1016/j.virol.2015.05.002 issn: 1096-0341 |
Appears in Collections: | (CBM) Artículos |
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Carrasco L Differential Action.pdf | 1,69 MB | Adobe PDF | ![]() View/Open |
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