English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/133519
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Differential action of pateamine A on translation of genomic and subgenomic mRNAs from Sindbis virus

AutorGonzález-Almela, Esther; Sanz, Miguel Ángel ; García-Moreno, Manuel ; Northcote, Peter; Pelletier, Jerry; Carrasco Llamas, Luis
Palabras claveAlphavirus protein synthesis
eIF4A Sindbis virus
Helicase inhibitor
Translation inhibitors
Fecha de publicación5-jun-2015
EditorElsevier
CitaciónVirology 484: 41- 50 (2015)
Resumen© 2015 Elsevier Inc. Pateamine A (Pat A) is a natural marine product that interacts specifically with the translation initiation factor eIF4A leading to the disruption of the eIF4F complex. In the present study, we have examined the activity of Pat A on the translation of Sindbis virus (SINV) mRNAs. Translation of genomic mRNA is strongly suppressed by Pat A, as shown by the reduction of nsP1 or nsP2 synthesis. Notably, protein synthesis directed by subgenomic mRNA is resistant to Pat A inhibition when the compound is added at late times following infection; however, subgenomic mRNA is sensitive to Pat A in transfected cells or in cell free systems, indicating that this viral mRNA exhibits a dual mechanism of translation. A detailed kinetic analysis of Pat A inhibition in SINV-infected cells demonstrates that a switch occurs approximately 4. h after infection, rendering subgenomic mRNA translation more resistant to Pat A inhibition.
URIhttp://hdl.handle.net/10261/133519
DOI10.1016/j.virol.2015.05.002
Identificadoresdoi: 10.1016/j.virol.2015.05.002
issn: 1096-0341
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Carrasco L Differential Action.pdf1,69 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.