English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/133157
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

African swine fever virus assembles a single membrane derived from rupture of the endoplasmic reticulum

AuthorsSuárez, Cristina; Andrés, Germán ; Kolovou, A; Hoppe, S.; Salas, María Luisa; Walther, P.; Krijnse Locker, J.
Issue Date24-Jul-2015
PublisherBlackwell Publishing
CitationCellular Microbiology 17: 1683- 1698 (2015)
AbstractCollective evidence argues that two members of the nucleocytoplasmic large DNA viruses (NCLDVs) acquire their membrane from open membrane intermediates, postulated to be derived from membrane rupture. We now study membrane acquisition of the NCLDV African swine fever virus. By electron tomography (ET), the virion assembles a single bilayer, derived from open membrane precursors that collect as ribbons in the cytoplasm. Biochemically, lumenal endoplasmic reticulum (ER) proteins are released into the cytosol, arguing that the open intermediates are ruptured ER membranes. ET shows that viral capsid assembles on the convex side of the open viral membrane to shape it into an icosahedron. The viral capsid is composed of tiny spikes with a diameter of ∼5nm, connected to the membrane by a 6nm wide structure displaying thin striations, as observed by several complementary electron microscopy imaging methods. Immature particles display an opening that closes after uptake of the viral genome and core proteins, followed by the formation of the mature virion. Together with our previous data, this study shows a common principle of NCLDVs to build a single internal envelope from open membrane intermediates. Our data now provide biochemical evidence that these open intermediates result from rupture of a cellular membrane, the ER. © 2015 John Wiley
Identifiersdoi: 10.1111/cmi.12468
issn: 1462-5822
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
Salas ML African Swine.pdf1,99 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.