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dc.contributor.authorCid, Cristinaen_US
dc.contributor.authorGarcía-Bonilla, Lidiaen_US
dc.contributor.authorCamafeita, Emilioen_US
dc.contributor.authorBurda, Jozefen_US
dc.contributor.authorSalinas, Matildeen_US
dc.contributor.authorAlcázar, Albertoen_US
dc.identifier.citationProteomics 7(17): 3207-3218 (2007)en_US
dc.description12 pages, 7 figures, 1 table.-- PMID: 17683050 [PubMed].-- ISI Article Identifier: 000249635200016.-- Printed version published Sep 2007.en_US
dc.description.abstractSerine/threonine protein phosphatase 1 (PP1) regulates multiple cellular processes. Protein phosphorylation-dephosphorylation is largely altered during ischemia and subsequent reperfusion. The brain is particularly vulnerable to stress resulting from ischemia-reperfusion (IR), however, the acquisition of ischemic tolerance (IT) protects against IR stress. We studied PP1 complexes in response to IR stress and IT in brain using proteomic characterization of PP1 complexes in animal models of IR and IT. PP1 α and PP1 γ were immunoprecipitated and resolved by 2-D. DIGE analysis detected 14 different PP1-interacting proteins that exhibited significant changes in their association with PP1 α or PP1 γ. These proteins were identified by MALDI-TOF MS. Seven had the PP1-binding RVxF motif. IR altered the interaction of heat shock cognate 71 kDa-protein, creatine kinase B, and dopamine- and cAMP-regulated phosphoprotein 32 kDa (DARPP32) with both PP1 α and PP1 γ, and the interaction of phosphodiesterase-6B, transitional ER ATPase, lamin-A, glucose-regulated 78 kDa-protein, dihydropyrimidinase-related protein-2, γ-enolase, neurofilament-L, and ubiquitin ligase SIAH2 with PP1 γ. IT prevented most of the IR-induced effects. This study identifies novel PP1-α and PP1 γ-interacting proteins and reveals an in vivo modularity of PP1 holoenzymes in response to physiological ischemic stress. It supports a potential role of PP1 in IR stress and as a target of the endogenous protective mechanisms induced by IT.-
dc.description.sponsorshipThis work was supported by Spanish Grants 05/ 1099 and 05/0312 from "Fondo de Investigación Sanitaria" and Retics – RD06/0026.-
dc.format.extent2373 bytes-
dc.publisherJohn Wiley & Sons-
dc.subjectIschemic tolerance-
dc.subjectProtein complexes-
dc.subjectProtein phosphatase 1-
dc.titleProteomic characterization of protein phosphatase 1 complexes in ischemia-reperfusion and ischemic toleranceen_US
dc.description.peerreviewedPeer revieweden_US
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