English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/132977
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Functional amyloids as inhibitors of plasmid DNA replication

AutorMolina-García, Laura ; Gasset-Rosa, F. ; Moreno-del Álamo, María ; Fernández-Tresguerres, M.E. ; Moreno Díaz de la Espina, Susana ; Lurz, Rudi; Giraldo, R.
Fecha de publicación5-may-2016
EditorNature Publishing Group
CitaciónScientific Reports 6: 25425 (2016)
ResumenDNA replication is tightly regulated to constrain the genetic material within strict spatiotemporal boundaries and copy numbers. Bacterial plasmids are autonomously replicating DNA molecules of much clinical, environmental and biotechnological interest. A mechanism used by plasmids to prevent over-replication is ‘handcuffing’, i.e. inactivating the replication origins in two DNA molecules by holding them together through a bridge built by a plasmid-encoded initiator protein (Rep). Besides being involved in handcuffing, the WH1 domain in the RepA protein assembles as amyloid fibres upon binding to DNA in vitro. The amyloid state in proteins is linked to specific human diseases, but determines selectable and epigenetically transmissible phenotypes in microorganisms. Here we have explored the connection between handcuffing and amyloidogenesis of full-length RepA. Using a monoclonal antibody specific for an amyloidogenic conformation of RepA-WH1, we have found that the handcuffed RepA assemblies, either reconstructed in vitro or in plasmids clustering at the bacterial nucleoid, are amyloidogenic. The replication-inhibitory RepA handcuff assembly is, to our knowledge, the first protein amyloid directly dealing with DNA. Built on an amyloid scaffold, bacterial plasmid handcuffs can bring a novel molecular solution to the universal problem of keeping control on DNA replication initiation.
Descripción8 p.-5 fig.
Versión del editorhttp://dx.doi.org/ 10.1038/srep25425
URIhttp://hdl.handle.net/10261/132977
DOI10.1038/srep25425
ISSN2045-2322
E-ISSN2045-2322
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
srep_2016.pdfArtículo principal2,36 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.