English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/132856
Título

Study of the interaction between the G-quadruplex-forming thrombin-binding aptamer and the porphyrin 5,10,15,20-tetrakis-(N-methyl-4-pyridyl)-21,23H-porphyrin tetratosylate

AutorToro, Miquel del; Gargallo, Raimundo; Eritja Casadellà, Ramón; Jaumot, Joaquim
Palabras claveChemometrics
Chromatography
DNA-drug interaction
G-quadruplex
Porphyrins
Fecha de publicación1-ago-2008
EditorAcademic Press
CitaciónAnalytical Biochemistry 379(1): 8-15(2008)
ResumenThe G-quadruplex DNA structure has been suggested to be a potential target for anticancer therapies. Therefore, there is increasing interest in the development of drugs that could modulate the stability of G-quadruplex structures. In the current work, the interaction between the thrombin-binding aptamer (TBA, 5′-GGT TGG TGT GGT TGG-3′), which can form an intramolecular G-quadruplex structure, and the porphyrin 5,10,15,20-tetrakis-(N-methyl-4-pyridyl)-21,23H-porphyrin tetratosylate (TmPyP4) was studied. The application of a high-performance liquid chromatography-photodiode array (HPLC-PDA) detector-based method to study this kind of interaction was tested. Molecular absorption data recorded along the chromatographic runs were analyzed by means of multivariate data analysis methods. Moreover, biospecific interaction analysis (BIA) by surface plasmon resonance (SPR) and melting and mole ratio experiments monitored by UV-visible molecular absorption and circular dichroism spectroscopies, were applied to confirm and expand the chromatographic studies. The results showed the formation of an interaction complex with a stoichiometry 1:1 (TmPyP4/TBA) and logarithm of the equilibrium constant equal to 5.7 ± 0.2. Melting and circular dichroism data reflected that the initial G-quadruplex structure of TBA is stabilized in the interaction complex, being slightly distorted by the presence of the ligand. © 2008 Elsevier Inc. All rights reserved.
Versión del editorhttp://dx.doi.org/10.1016/j.ab.2008.04.044
URIhttp://hdl.handle.net/10261/132856
DOI10.1016/j.ab.2008.04.044
Aparece en las colecciones: (IQAC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
AnalBiochemTBA2008.pdf708,49 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.