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dc.contributor.authorKöber, Mariana-
dc.contributor.authorMoros, María-
dc.contributor.authorFranco Fraguas, Laura-
dc.contributor.authorGrazú, Valeria-
dc.contributor.authorFuente, Jesús M. de la-
dc.contributor.authorLuna, Mónica-
dc.contributor.authorBriones Fernández-Pola, Fernando-
dc.identifierissn: 1520-6882-
dc.identifier.citationAnalytical Chemistry 86(24): 12159-12165 (2014)-
dc.description.abstract© 2014 American Chemical Society. The development of sensitive and easy-to-use biosensors that allow an adequate characterization of specific weak biological interactions like carbohydrate-lectin interactions still remains challenging today. Nanoparticles functionalized with carbohydrates are one of the most powerful systems for studying carbohydrate-lectin interactions, because they mimic the multivalent presentation of carbohydrates encountered in nature, for example when viruses and bacteria bind to cells. On the basis of the model system glucose-Concanavalin A (ConA), we explore the application of Transient Magnetic Birefringence (TMB) to study these weak interactions, using glucose-functionalized colloidal magnetite nanoparticles (NPs) as probes. We demonstrate that the binding dynamics can be monitored and derive a model to obtain the apparent cooperativity. For our studies, we use nanoparticles of 6 and 8 nm in diameter. The ConA-generated response shows apparent cooperativity, due to the cross-linking of nanoparticles by the ConA tetramer which has four binding sites. Cooperativity is higher for 6 nm NPs, possibly due to a better accessibility of all four ConA binding sites on smaller NPs, enhancing cross-linking. For this system, we find a detection limit of 3-23 nM. (Chemical Equation Presented).-
dc.description.sponsorshipThis work has been supported by the Spanish Ministry of Education and Science (NAN2004-09125-C07-02), the Spanish National Research Council (projects 200550F0172 and 2009UY0024), the Spanish Ministry of Industry (FIT-010000-2006-98), the Spanish Ministry of Science and Innovation (CTQ2008-03739/PPQ), and the European Research Council (Starting Grant NANOPUZZLE). M.K. gratefully acknowledges support through a PhD fellowship I3P financed by the Spanish National Research Council and the European Commission. M.M. acknowledges financial support from the Spanish Ministry of Economy and Competitiveness (CONSOLIDER-NANOBIOMED).-
dc.publisherAmerican Chemical Society-
dc.titleNanoparticle-mediated monitoring of carbohydrate-lectin interactions using transient magnetic birefringence-
dc.description.versionPeer Reviewed-
dc.contributor.funderMinisterio de Educación y Ciencia (España)-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderMinisterio de Industria, Energía y Turismo (España)-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderEuropean Research Council-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderEuropean Commission-
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