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Nanoparticle-mediated monitoring of carbohydrate-lectin interactions using transient magnetic birefringence

AutorKöber, Mariana; Moros, María; Franco Fraguas, Laura; Grazú, Valeria; Fuente, Jesús M. de la; Luna, Mónica ; Briones Fernández-Pola, Fernando
Fecha de publicación2014
EditorAmerican Chemical Society
CitaciónAnalytical Chemistry 86(24): 12159-12165 (2014)
Resumen© 2014 American Chemical Society. The development of sensitive and easy-to-use biosensors that allow an adequate characterization of specific weak biological interactions like carbohydrate-lectin interactions still remains challenging today. Nanoparticles functionalized with carbohydrates are one of the most powerful systems for studying carbohydrate-lectin interactions, because they mimic the multivalent presentation of carbohydrates encountered in nature, for example when viruses and bacteria bind to cells. On the basis of the model system glucose-Concanavalin A (ConA), we explore the application of Transient Magnetic Birefringence (TMB) to study these weak interactions, using glucose-functionalized colloidal magnetite nanoparticles (NPs) as probes. We demonstrate that the binding dynamics can be monitored and derive a model to obtain the apparent cooperativity. For our studies, we use nanoparticles of 6 and 8 nm in diameter. The ConA-generated response shows apparent cooperativity, due to the cross-linking of nanoparticles by the ConA tetramer which has four binding sites. Cooperativity is higher for 6 nm NPs, possibly due to a better accessibility of all four ConA binding sites on smaller NPs, enhancing cross-linking. For this system, we find a detection limit of 3-23 nM. (Chemical Equation Presented).
Versión del editorhttp://dx.doi.org/10.1021/ac503122y
URIhttp://hdl.handle.net/10261/132518
DOI10.1021/ac503122y
Identificadoresissn: 1520-6882
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