Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/132490
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Human bone-lineage cell responses to anisotropic Ti6Al4V surfaces are dependent on their maturation state

AutorCalzado-Martín, Alicia CSIC; Crespo, Lara; Saldaña, Laura; Boré, Alba; Gómez Barrena, Enrique; Vilaboa, N.
Palabras claveSurface topography
Titanium alloy
Mesenchymal stem cell
Osteoblast
Cell signalling
Fecha de publicaciónsep-2014
EditorJohn Wiley & Sons
CitaciónJournal of Biomedical Materials Research - Part A 102(9): 3154-3166 (2014)
ResumenThis article reports on the interactions of human bone cells, mesenchymal stem cells (hMSCs) from bone marrow and osteoblasts (hOBs), with a submicron-grooved Ti6Al4V alloy that promotes cell orientation in the direction of the anisotropy. Adhesion sites, actin and tubulin networks and fibronectin extracellular matrix of both cell types align with the direction of the grooves. hMSCs adhere at a higher rate on the patterned substrate than on the polished alloy, while no differences are found in hOBs attachment. Compared to the flat substrate, RhoA activity is higher in hMSCs and hOB cultured on the grooved alloy and treatment with C3 transferase leads to loss of organization of actin and tubulin cytoskeletons. Rho-associated kinase (ROCK) activity of hMSCs is upregulated on the anisotropic samples, but not affected in hOBs. Treatment with hydroxyfasudil disrupts the alignment of adhesion sites in hMSCs but not in hOBs. When cells are cultured in media that support osteogenic maturation, OPN secretion increases in hMSCs on the anisotropic alloy and it remains unaffected in hOBs. Cell layer calcification proceeds to a same extent in hMSCs cultured on the two metallic surfaces but decreases in hOBs cultured on the patterned samples. Taken together, these results indicate that hOBs are less sensitive than hMSCs to the patterned Ti6Al4V alloy. This effect can be attributed to their different stages of cell maturation and may be mediated, at least in part, through ROCK signaling because its activity increases on hMSCs cultured on the patterned alloy, while hOBs fail to upregulate it. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3154-3166, 2014. © 2013 Wiley Periodicals, Inc.
Versión del editorhttp://dx.doi.org/10.1002/jbm.a.34987
URIhttp://hdl.handle.net/10261/132490
DOI10.1002/jbm.a.34987
Identificadorese-issn: 1552-4965
issn: 1549-3296
Aparece en las colecciones: (IMN-CNM) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

5
checked on 19-mar-2024

WEB OF SCIENCETM
Citations

3
checked on 27-feb-2024

Page view(s)

225
checked on 29-mar-2024

Download(s)

147
checked on 29-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.