English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/132082
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Functional coexistence of twin arsenic resistance systems in Pseudomonas putida KT2440

AuthorsPáez-Espino, A. David; Durante-Rodríguez, Gonzalo ; Lorenzo, Víctor de
KeywordsPseudomonas putida
Arsenic
Detoxification
Phosphate
Tmk
Issue DateJan-2015
PublisherBlackwell Publishing
CitationEnvironmental microbiology, 17(1) : 229-238 (2015)
AbstractThe genome of the soil bacterium Pseudomonas putida KT2440 bears two virtually identical arsRBCH operons putatively encoding resistance to inorganic arsenic species. Single and double chromosomal deletions in each of these ars clusters of this bacterium were tested for arsenic sensitivity and found that the contribution of each operon to the resistance to the metalloid was not additive, as either cluster sufficed to endow cells with high-level resistance. However, otherwise identical traits linked to each of the ars sites diverged when temperature was decreased. Growth of the various mutants at 15°C (instead of the standard 30°C for P. putida) uncovered that ars2 affords a much higher resistance to As (III) than the ars1 counterpart. Reverse transcription polymerase chain reaction of arsB1 and arsB2 genes as well as lacZ fusions to the Pars1 and Pars2 promoters traced the difference to variations in transcription of the corresponding gene sets at each temperature. Functional redundancy may thus be selected as a stable condition - rather than just as transient state - if it affords one key activity to be expressed under a wider range of physicochemical settings. This seems to provide a straightforward solution to regulatory problems in environmental bacteria that thrive under changing scenarios.
Publisher version (URL)http://dx.doi.org/10.1111/1462-2920.12464
URIhttp://hdl.handle.net/10261/132082
DOI10.1111/1462-2920.12464
ISSN1462-2912
E-ISSN1462-2920
Appears in Collections:(CNB) Artículos
Files in This Item:
File Description SizeFormat 
12.302_Functional_Paez-Espino et al 2014.pdf5,06 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.