English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/131922
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Soybean Ferritin Expression in Saccharomyces cerevisiae Modulates Iron Accumulation and Resistance to Elevated Iron Concentrations

Autorde Llanos, Rosa ; Martínez Garay, Carlos Andrés; Fita Torró, Josep; Romero, Antonia ; Martínez Pastor, M. Teresa; Puig, Sergi
Palabras claveSaccharomyces cerevisiae
Ferritin
Iron deficiency
Yeast
Iron metabolism
SFerH1
SFerH2
Fecha de publicaciónmar-2016
EditorAmerican Society for Microbiology
CitaciónApplied and Environmental Microbiology 82 (10): 3052-3060 (2016)
ResumenFungi, including the yeast Saccharomyces cerevisiae, lack ferritin and use vacuoles as iron storage organelles. This work explored how plant ferritin expression influenced baker's yeast iron metabolism. Soybean seed ferritin H1 (SFerH1) and SFerH2 genes were cloned and expressed in yeast cells. Both soybean ferritins assembled as multimeric complexes, which bound yeast intracellular iron in vivo and, consequently, induced the activation of the genes expressed during iron scarcity. Soybean ferritin protected yeast cells that lacked the Ccc1 vacuolar iron detoxification transporter from toxic iron levels by reducing cellular oxidation, thus allowing growth at high iron concentrations. Interestingly, when simultaneously expressed in ccc1Δ cells, SFerH1 and SFerH2 assembled as heteropolymers, which further increased iron resistance and reduced the oxidative stress produced by excess iron compared to ferritin homopolymer complexes. Finally, soybean ferritin expression led to increased iron accumulation in both wild-type and ccc1Δ yeast cells at certain environmental iron concentrations.
Versión del editorhttp://dx.doi.org/10.1128/AEM.00305-16
URIhttp://hdl.handle.net/10261/131922
DOI10.1128/AEM.00305-16
ISSN0099-2240
Aparece en las colecciones: (IATA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
AEM-2016-Llanos.pdfArtículo principal554,03 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.