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Schistosome infections induce significant changes in the host biliary proteome

AutorTorre Escudero, Eduardo de la ; Pérez Sánchez, Ricardo ; Manzano Román, Raúl ; Oleaga, Ana
Palabras claveSchistosoma bovis
Fecha de publicación2015
CitaciónJournal of Proteomics 114(1): 71-82 (2015)
ResumenSchistosomiasis is a disease caused by blood trematodes affecting man and animals that represents an important human health and veterinary problem. Main damages caused by this infection are a consequence of the host inflammatory reaction against the parasite eggs trapped inside the liver. Despite the hepatic pathology of schistosomiasis is very well known, there is no specific studies dealing with the schistosome infection effects on the biliary function. The purpose of this work was to analyse the changes induced by Schistosoma bovis infection in the biliary proteome. For this, whole gallbladders from S. bovis-infected and non-infected mice were dissected, homogenized and fractionated by differential centrifugation. The resulting protein fractions were resolved by SDS-PAGE, the gels were sliced, and the gel pieces analyzed by LC-MS/MS. Altogether, we identified 1,937 proteins, which were classified according to their “protein class” and “molecular function”, and then subjected to an“Enrichment analysis”. The differences found in gallbladder proteomes between S. bovis-infected and non-infected mice are analysed. We show that chronic schistosome infections cause significant changes in the biliary proteome that may produce physiological alterations and affect the therapeutic actions of drugs when administered to human patients and animals with schistosomiasis
Descripción36 páginas, 5 figuras, 2 tablas. -- The definitive version is available at http://www.elsevier.com. -- The authors are grateful to Rocío Vizcaíno Marín, from the Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC) (Spain), for her skillful technical assistance, and to Dr. Luz Valero, from the Proteomics Unit of the University of Valencia (Spain), for her assistance in the MS analyses.
Versión del editorhttp://dx.doi.org/10.1016/j.jprot.2014.11.009
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