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Study of the bacterial biodiversity in table olives

AutorMedina Pradas, Eduardo ; Romero-Gil, Verónica; Landa, Blanca B. ; Montes Borrego, Miguel ; Ruiz-Bellido, M.; Arroyo López, Francisco Noé
Fecha de publicaciónoct-2015
Citación3rd International Conference on Microbial Diversity (2015)
ResumenThe objective of this research was to characterize the biochemistry and microbiology of spontaneous PDO Aloreña de Málaga table olive fermentations and define the bacterial biodiversity during the process. The study was carried out in two different locations in Malaga province (Spain) following the fermentation during 4 months. Biochemical analysis of olives showed a progressive increase in free acidity as consequent of a spontaneous fermentation, with pH and salt values characteristic of this type of product. Both locations showed a similar trend in microbiology enumeration by plating in selective media. Yeasts were the most abundant microorganisms during the time, while lactic acid bacteria (LAB) cell count revealed low concentration or even were not detected. The novel of this research is the study of the microbial ecology of olives fermentation using bar-coded pyrosequencing of 16S rDNA to characterize bacterial diversity. The bacterial α- and β- diversity differed between fruits and brines being higher for fruits especially at the beginning of the fermentation process and the opposite for the brines. Bacterial diversity changed during fermentation time, and a total of 323 and 150 operational taxonomic units (OTUs) were identified in fruits and brines, respectively. The predominant genera were Celerinantantimonas, Pseudomonas, Propionibacterium, and diverse Acetobacteraceae. Some differences in the bacterial diversity were found between industries, special at the beginning of the process, whereas no differences were found for the rest of physicochemical parameters such as color and texture in the final product.
DescripciónTrabajo presentado en la 3rd International Conference on Microbial Diversity (MD2015, "The Challenge of Complexity"), celebrada en Perugia del 27 al 29 de octubre de 2015.
URIhttp://hdl.handle.net/10261/131872
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