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DNA melting within a binary σ(54)-promoter DNA complex

AutorCannon, Wendy; Gallegos, María Trinidad ; Buck, Martin
Palabras claveDNA melting
Transcription activation
Fecha de publicación5-ene-2001
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 276(1): 386-394 (2001)
ResumenThe σ(54) subunit of the bacterial RNA polymerase requires the action of specialized enhancer-binding activators to initiate transcription. Here we show that σ(54) is able to melt promoter DNA when it is bound to a DNA structure representing the initial nucleation of DNA opening found in closed complexes. Melting occurs in response to activator in a nucleotide-hydrolyzing reaction and appears to spread downstream from the nucleation point toward the transcription start site. We show that σ(54) contains some weak determinants for DNA melting that are masked by the Region I sequences and some strong ones that require Region I. It seems that σ(54) binds to DNA in a self-inhibited state, and one function of the activator is therefore to promote a conformational change in σ(54) to reveal its DNA-melting activity. Results with the holoenzyme bound to early melted DNA suggest an ordered series of events in which changes in core to σ(54) interactions and σ(54)-DNA interactions occur in response to activator to allow σ(54) isomerization and the holoenzyme to progress from the closed complex to the open complex.
Descripción9 pages, 7 figures, 1 table.-- PMID: 11036081 [PubMed].-- Available online Oct 17, 2000.-- Full-text version available Open Access at the journal site.
Versión del editorhttp://dx.doi.org/10.1074/jbc.M007779200
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