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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/131707
Título

Synthesis of potentially-bioactive lactosyl-oligofructosides by a novel bi-enzymatic system using bacterial fructansucrases

AutorDíez-Municio, M. ; González Santana, Clara; Rivas, Blanca de las ; Jimeno, M. Luisa ; Muñoz, Rosario ; Moreno, F. Javier ; Herrero, Miguel
Palabras claveLactosyl-oligofructosides
Lactosucrose
Inulosucrase
Levansucrase
Bi-enzymatic system
Prebiotic
Transfructosylation
Fecha de publicacióndic-2015
EditorElsevier
CitaciónFood Research International 78: 258–265 (2015)
ResumenEfficient enzymatic synthesis of lactosyl-oligofructosides (LFOS) with a degree of polymerization from 4 to 8 was achieved in the presence of sucrose:lactosucrose and sucrose:lactose mixtures by transfructosylation reaction. The main synthesized LFOS which consist of β-2,1-linked fructose to lactosucrose: β-d-galactopyranosyl-(1 → 4)-α-d-glucopyranosyl-[(1 → 2)-β-d-fructofuranosyl]n-(1 → 2)-β-d-fructofuranoside (where n refers to the number of transferred fructose moieties) was structurally characterized by nuclear magnetic resonance (NMR). The maximum formation of LFOS was 81% (in weight with respect to the initial amount of lactosucrose) and was obtained after 24 h of transfructosylation reaction based on sucrose:lactosucrose (250 g L− 1 each) catalyzed by an inulosucrase from Lactobacillus gasseri DSM 20604 (IS). The production of LFOS in the presence of sucrose:lactose mixtures required a previous high-yield lactosucrose synthesis step catalyzed by using a levansucrase from Bacillus subtilis CECT 39 (LS) before the inulosucrase-catalyzed reaction. This novel one-pot bi-enzymatic system led to the synthesis of about 22% LFOS in weight, with respect to the initial amount of lactose (250 g L− 1). The results revealed a high specificity for the substrate involved in the inulosucrase-catalyzed reaction given that, although lactosucrose (O-β-d-galactopyranosyl-(1 → 4)-O-α-d-glucopyranosyl-(1 → 2)-β-d-fructofuranoside) acted as a strong acceptor of β-2,1-linked fructose, lactose (β-d-galactopyranosyl-(1 → 4)-α-d-glucose) was found to be an extremely weak acceptor.
Versión del editorhttp://dx.doi.org/10.1016/j.foodres.2015.09.035
URIhttp://hdl.handle.net/10261/131707
DOI10.1016/j.foodres.2015.09.035
ISSN0963-9969
E-ISSN1873-7145
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