English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/131705
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

The private life of environmental bacteria: pollutant biodegradation at the single cell level

AuthorsNikel, Pablo I.; Silva-Rocha, Rafael; Benedetti, Ilaria; Lorenzo, Víctor de
KeywordsStochastic process
Pseudomonas putida
TOL plasmid
Biodegradation
Transcriptional regulation
Flow cytometry
Raman spectroscopy
Issue DateMar-2014
PublisherBlackwell Publishing
CitationEnvironmental Microbiology, 16(3) : 628-642 (2014)
AbstractBacteria display considerable cell-to-cell heterogeneity in a number of genetic and physiological traits. Stochastic differences in regulatory patterns (e.g., at the transcriptional level) propagate into the metabolic and physiological status of otherwise isogenic cells, which ultimately results in appearance of sub-populations within the community. As new technologies emerge and because novel single cell strategies are constantly being refined, our knowledge on microbial individuality is in burgeoning and constant expansion. These approaches encompass not only molecular biology tools (e.g., fluorescent protein based reporters) but also a suite of sophisticated, non-invasive technologies to gain insight into the metabolic state of individual cells. Defining the role of individual heterogeneities is thus instrumental for the population-level understanding of macroscopic processes, in both environmental and industrial setups. The present article reviews the state-of-the-art methodologies for the investigation of single bacteria at both the genetic and metabolic level, and places the application of currently available tools in the context of microbial ecology and environmental microbiology. As a case example, we examine the stochastic and multi-stable behaviour of the TOL-encoded pathway of Pseudomonas putida mt-2 for the biodegradation of aromatic compounds. Bet-hedging strategies and division of labour are considered as factors pushing forward the evolution of environmental microorganisms.
Publisher version (URL)http://dx.doi.org/10.1111/1462-2920.12360
URIhttp://hdl.handle.net/10261/131705
DOI10.1111/1462-2920.12360
ISSN1462-2912
E-ISSN1462-2920
Appears in Collections:(CNB) Artículos
Files in This Item:
File Description SizeFormat 
7.290_The private life_Nikel et al 2014-1.pdfArtículo principal2,24 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.