English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/131705
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

The private life of environmental bacteria: pollutant biodegradation at the single cell level

AutorNikel, Pablo I.; Silva-Rocha, Rafael; Benedetti, Ilaria; Lorenzo, Víctor de
Palabras claveStochastic process
Pseudomonas putida
TOL plasmid
Transcriptional regulation
Flow cytometry
Raman spectroscopy
Fecha de publicaciónmar-2014
EditorBlackwell Publishing
CitaciónEnvironmental Microbiology, 16(3) : 628-642 (2014)
ResumenBacteria display considerable cell-to-cell heterogeneity in a number of genetic and physiological traits. Stochastic differences in regulatory patterns (e.g., at the transcriptional level) propagate into the metabolic and physiological status of otherwise isogenic cells, which ultimately results in appearance of sub-populations within the community. As new technologies emerge and because novel single cell strategies are constantly being refined, our knowledge on microbial individuality is in burgeoning and constant expansion. These approaches encompass not only molecular biology tools (e.g., fluorescent protein based reporters) but also a suite of sophisticated, non-invasive technologies to gain insight into the metabolic state of individual cells. Defining the role of individual heterogeneities is thus instrumental for the population-level understanding of macroscopic processes, in both environmental and industrial setups. The present article reviews the state-of-the-art methodologies for the investigation of single bacteria at both the genetic and metabolic level, and places the application of currently available tools in the context of microbial ecology and environmental microbiology. As a case example, we examine the stochastic and multi-stable behaviour of the TOL-encoded pathway of Pseudomonas putida mt-2 for the biodegradation of aromatic compounds. Bet-hedging strategies and division of labour are considered as factors pushing forward the evolution of environmental microorganisms.
Versión del editorhttp://dx.doi.org/10.1111/1462-2920.12360
Aparece en las colecciones: (CNB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
7.290_The private life_Nikel et al 2014-1.pdfArtículo principal2,24 MBAdobe PDFVista previa
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.