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Título

Highly sensitive dendrimer-based nanoplasmonic biosensor for drug allergy diagnosis

AutorSoler, María ; Estévez, M. Carmen ; Otte, Marinus A. ; Sepúlveda, Borja ; Lechuga, Laura M.
Palabras claveDendrimer
IgE quantification
Label-free detection
Allergy diagnosis
Serum clinical sample
Nanoplasmonic biosensor
Gold nanodisks
Fecha de publicación2015
EditorElsevier
CitaciónBiosensors and Bioelectronics 66: 115-123 (2015)
ResumenA label-free biosensing strategy for amoxicillin (AX) allergy diagnosis based on the combination of novel dendrimer-based conjugates and a recently developed nanoplasmonic sensor technology is reported. Gold nanodisks were functionalized with a custom-designed thiol-ending-polyamido-based dendron (d-BAPAD) peripherally decorated with amoxicilloyl (AXO) groups (d-BAPAD–AXO) in order to detect specific IgE generated in patient's serum against this antibiotic during an allergy outbreak. This innovative strategy, which follows a simple one-step immobilization procedure, shows exceptional results in terms of sensitivity and robustness, leading to a highly-reproducible and long-term stable surface which allows achieving extremely low limits of detection. Moreover, the viability of this biosensor approach to analyze human biological samples has been demonstrated by directly analyzing and quantifying specific anti-AX antibodies in patient's serum without any sample pretreatment. An excellent limit of detection (LoD) of 0.6 ng/mL (i.e. 0.25 kU/L) has been achieved in the evaluation of clinical samples evidencing the potential of our nanoplasmonic biosensor as an advanced diagnostic tool to quickly identify allergic patients. The results have been compared and validated with a conventional clinical immunofluorescence assay (ImmunoCAP test), confirming an excellent correlation between both techniques. The combination of a novel compact nanoplasmonic platform and a dendrimer-based strategy provides a highly sensitive label free biosensor approach with over two times better detectability than conventional SPR. Both the biosensor device and the carrier structure hold great potential in clinical diagnosis for biomarker analysis in whole serum samples and other human biological samples.
URIhttp://hdl.handle.net/10261/131693
DOI10.1016/j.bios.2014.10.081
Identificadoresdoi: 10.1016/j.bios.2014.10.081
issn: 0956-5663
e-issn: 1873-4235
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