English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/131652
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Lysine-based surfactants in nanovesicle formulations: the role of cationic charge position and hydrophobicity in in vitro cytotoxicity and intracellular delivery

AuthorsNogueira, Daniele Rubert; Morán, Maria del Carmen; Mitjans, Montserrat; Pérez, Lourdes F.; Ramos, David L.; De Lapuente, Joaquín; Vinardell, María Pilar
KeywordsCell culture
Cell internalisation
Drug delivery
Issue Date4-Jun-2014
PublisherInforma Healthcare
CitationNanotoxicology 8(4): 404-421(2014)
AbstractUnderstanding nanomaterial interactions within cells is of increasing importance for assessing their toxicity and cellular transport. Here, the authors developed nanovesicles containing bioactive cationic lysine-based amphiphiles and assessed whether these cationic compounds increase the likelihood of intracellular delivery and modulate toxicity. Different cytotoxic responses were found among the formulations, depending on surfactant, cell line and endpoint assayed. The induction of mitochondrial dysfunction, oxidative stress and apoptosis were the general mechanisms underlying cytotoxicity. Fluorescence microscopy analysis demonstrated that nanovesicles were internalised by HeLa cells and evidenced that their ability to release endocytosed materials into cell cytoplasm depends on the structural parameters of amphiphiles. The cationic charge position and hydrophobicity of surfactants determine the nanovesicle interactions within the cell and, thus, the resulting toxicity and intracellular behaviour after cell uptake of the nanomaterial. The insights into some toxicity mechanisms of these new nanomaterials contribute in reducing the uncertainty surrounding their potential health hazards. © 2014 Informa UK, Ltd.
Publisher version (URL)http://dx.doi.org/10.3109/17435390.2013.793779
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
2014_Nanotoxicology.doc16,53 MBMicrosoft WordView/Open
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.