English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/13162
Título

Binding of transcriptional activators to sigma 54 in the presence of the transition state analog ADP-aluminum fluoride: insights into activator mechanochemical action

AutorChaney, Matthew; Grande, Ricardo; Wigneshweraraj, Siva R.; Cannon, Wendy; Casaz, Paul; Gallegos, María Trinidad ; Schumacher, Jorg; Jones, Susan; Elderkin, Sarah; Dago, Angel Ernesto; Morett, Enrique; Buck, Martin
Palabras claveActivators
Sigma 54
ADP · AlFx
AAA+ proteins
Fecha de publicación11-jul-2001
EditorCold Spring Harbor Laboratory. Press
CitaciónGenes and Development 15(17):2282-94
ResumenConformational changes in sigma 54(σ54) and σ54-holoenzyme depend on nucleotide hydrolysis by an activator. We now show that σ54 and its holoenzyme bind to the central ATP-hydrolyzing domains of the transcriptional activators PspF and NifA in the presence of ADP–aluminum fluoride, an analog of ATP in the transition state for hydrolysis. Direct binding of σ54 Region I to activator in the presence of ADP–aluminum fluoride was shown and inferred from in vivo suppression genetics. Energy transduction appears to occur through activator contacts to σ54 Region I. ADP–aluminum fluoride-dependent interactions and consideration of other AAA+ proteins provide insight into activator mechanochemical action.
Versión del editorhttp://dx.doi.org/10.1101/gad.205501
URIhttp://hdl.handle.net/10261/13162
DOI10.1101/gad.205501
ISSN2282–2294
Aparece en las colecciones: (EEZ) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.