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dc.contributor.authorRojas, Antonia-
dc.contributor.authorSegura, Ana-
dc.contributor.authorGuazzaroni, María Eugenia-
dc.contributor.authorTerán, Wilson-
dc.contributor.authorHurtado, Ana-
dc.contributor.authorGallegos, María Trinidad-
dc.contributor.authorRamos, Juan L.-
dc.identifier.citationJournal of Bacteriology 2003 Aug;185(16):4755-63en_US
dc.description.abstractThe TtgGHI efflux pump of Pseudomonas putida DOT-T1E plays a key role in the innate and induced tolerance of this strain to aromatic hydrocarbons and antibiotics. The ttgGHI operon is expressed constitutively from two overlapping promoters in the absence of solvents and at a higher level in their presence, but not in response to antibiotics. Adjacent to the ttgGHI operon is the divergently transcribed ttgVW operon. In TtgVdeficient backgrounds, although not in a TtgW-deficient background, expression of the ttgGHI and ttgVW operons increased fourfold. This suggests that TtgV represses expression from the ttgG promoters and controls its own. TtgW plays no major role in the regulation of expression of these promoters. Primer extension revealed that the divergent ttgG and ttgV promoters overlap, and mobility shift assays indicated that TtgV binds to this region with high affinity. DNaseI footprint assays revealed that TtgV protected four DNA helical turns that include the -10 and -35 boxes of the ttgV and ttgG promotersen_US
dc.description.sponsorshipThis study was supported by a grant from the European Commission (QLK3-CT-2001-00435) and a grant from the Comisión Interministerial de Ciencia y Tecnología (BIO 2003-00515 CICYT-2003).en_US
dc.format.extent579917 bytes-
dc.publisherAmerican Society for Microbiologyen_US
dc.subjectPseudomonas putidaen_US
dc.titleIn vivo and in vitro evidence that TtgV is the specific regulator of the TtgGHI multidrug and solvent efflux pump of Pseudomonas putidaen_US
dc.description.peerreviewedPeer revieweden_US
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