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dc.contributor.author | Nikel, Pablo I. | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Lorenzo, Víctor de | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Environmental Microbiology 16: 239- 254 (2014) | - |
dc.identifier.issn | 1462-2912 | - |
dc.identifier.uri | http://hdl.handle.net/10261/131448 | - |
dc.description.abstract | While the natural niches of the soil bacterium Pseudomonas putida are unlikely to include significant amounts of free glycerol as a growth substrate, this bacterium is genetically equipped with the functions required for its metabolism. We have resorted to deep sequencing of the transcripts in glycerol-grown P.putidaKT2440 cells to gain an insight into the biochemical and regulatory components involved in the shift between customary C sources (e.g. glucose or succinate) to the polyol. Transcriptomic results were contrasted with key enzymatic activities under the same culture conditions. Cognate expression profiles revealed that genes encoding enzymes of the Entner-Doudoroff route and other catabolic pathways, e.g. the gluconate and 2-ketogluconate loops, were significantly downregulated on glycerol. Yet, the compound simultaneously elicited a gluconeogenic response that indicated an efficient channelling of C skeletons back to biomass build-up through the glyoxylate shunt rather than energization of the cells through downwards pathways, i.e. tricarboxylic acid cycle and oxidative phosphorylation. The simultaneous glycolytic and gluconeogenic metabolic regimes on glycerol, paradoxical as they seem, make sense from an ecological point of view by favouring prevalence versus exploration. This metabolic situation was accompanied by a considerably low expression of stress markers as compared with other C sources. © 2013 Society for Applied Microbiology and John Wiley & Sons Ltd. | - |
dc.description.sponsorship | This study was supported by the BIO and FEDER CONSOLIDER-INGENIO programmeof the Spanish Ministry of Science and Innovation, the MICROME, ST-FLOW and ARISYS Contracts of the EU, and the PROMT Project of the CAM. PIN is a researcher from the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina) and holds a Marie Curie Actions Program grant from the EC (ALLEGRO, UE-FP7-PEOPLE-2011-IIF-300508). JK is a beneficiary of the predoctoral JAE Program of the CSIC. | - |
dc.publisher | Blackwell Publishing | - |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | - |
dc.subject | Bacterial physiology | - |
dc.subject | Glycerol | - |
dc.subject | Pseudomonas putida. | - |
dc.title | Metabolic and regulatory rearrangements underlying glycerol metabolism in Pseudomonas putidaKT2440 | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1111/1462-2920.12224 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1111/1462-2920.12224 | - |
dc.identifier.e-issn | 1462-2920 | - |
dc.date.updated | 2016-04-26T09:35:32Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.relation.csic | Sí | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (CNB) Artículos |
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2.283_Metabolic and regulatory_Nikel et al 2013.pdf | 2,55 MB | Adobe PDF | Visualizar/Abrir |
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