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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Nogueira, Daniele Rubert | es_ES |
dc.contributor.author | Tavano, Lorena | es_ES |
dc.contributor.author | Mitjans, Montserrat | es_ES |
dc.contributor.author | Pérez, Lourdes | es_ES |
dc.contributor.author | Infante, María Rosa | es_ES |
dc.contributor.author | Vinardell, M. Pilar | es_ES |
dc.date.accessioned | 2016-04-25T10:15:50Z | - |
dc.date.available | 2016-04-25T10:15:50Z | - |
dc.date.issued | 2013-04 | - |
dc.identifier.citation | Biomaterials | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/131389 | - |
dc.description.abstract | Nanoparticles with pH-sensitive behavior may enhance the success of chemotherapy in many cancers by efficient intracellular drug delivery. Here, we investigated the effect of a bioactive surfactant with pH-sensitive properties on the antitumor activity and intracellular behavior of methotrexate-loaded chitosan nanoparticles (MTX-CS-NPs). NPs were prepared using a modified ionotropic complexation process, in which was included the surfactant derived from Nα,Nε-dioctanoyl lysine with an inorganic lithium counterion. The pH-sensitive behavior of NPs allowed accelerated release of MTX in an acidic medium, as well as membrane-lytic pH-dependent activity, which facilitated the cytosolic delivery of endocytosed materials. Moreover, our results clearly proved that MTX-CS-NPs were more active against the tumor HeLa and MCF-7 cell lines than the free drug. The feasibilty of using NPs to target acidic tumor extracellular pH was also shown, as cytotoxicity against cancer cells was greater in a mildly acidic environment. Finally, the combined physicochemical and pH-sensitive properties of NPs generally allowed the entrapped drug to induce greater cell cycle arrest and apoptotic effects. Therefore, our overall results suggest that pH-sensitive MTX-CS-NPs could be potentially useful as a carrier system for tumor and intracellular drug delivery in cancer therapy. © 2013 Elsevier Ltd | es_ES |
dc.description.sponsorship | This research was supported by Projects CTQ2009-14151-C02-02 and CTQ2009-14151-C02-01 of the Ministerio de Ciencia e Innovación (Spain). Daniele Rubert Nogueira holds a PhD grant from MAEC-AECID (Spain). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.isversionof | Postprint | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Chitosan nanoparticles | es_ES |
dc.subject | Cytotoxicity | es_ES |
dc.subject | Intracellular drug delivery | es_ES |
dc.subject | Methotrexate | es_ES |
dc.subject | PH-sensitivity | es_ES |
dc.subject | Lysine-based surfactant | es_ES |
dc.title | In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.biomaterials.2013.01.005 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.biomaterials.2013.01.005 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
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