English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/13118
Título

Advances in the ultrastructural study of the implant–bone interface by backscattered electron imaging.

AutorWierzchos, Jacek ; Falcioni, T.; Kiciak, A.; Wolin´ski, J.; Koczorowski, R.; Chomicki, P.; Porembska, M.; Ascaso, Carmen
Palabras claveAnimal study
Bone cells
Bone formation
Implants
Interface
Osseointegration
SEM-BSE
Fecha de publicaciónene-2008
EditorElsevier
CitaciónMicron 39 (2008) 1363–1370
ResumenThe biocompatibility of titanium implants in bone depends on the response shown by cells in contact with the implant surface. Several developments have been targeted at achieving successful implant treatment. The aim of this study was to develop a novel preparation procedure to evaluate the bone cell response produced at the bone–implant interface using the technique scanning electron microscopy with backscattered electron imaging (SEM-BSE). Dental prostheses with an SLA-modified or TOP-modified surface were implanted in a toothless part of the mandibula in female pigs. The animals were sacrificed 12 weeks after surgery, at which time block specimens containing the implants were obtained. These specimens were then processed for SEM-BSE by optimizing a protocol involving chemical fixation and heavy metal staining. In addition, element distribution maps for the implant–bone tissue interface were obtained using a microanalytical system based on energy-dispersive X-ray spectrometry (EDS). This novel visualisation approach enabled a comprehensive study of the extracellular matrix and cell components of the host tissues neoformed around the implant. SEM-BSE images also provided ultrastructural details of the bone cells. This technique appears to be an effective and very promising tool for detailed studies on the implant–bone tissue interface and the host response to the bone incorporation process
Descripción8 p. 3 con fotos
Versión del editorhttp://dx.doi.org/10.1016/j.micron.2008.01.022
URIhttp://hdl.handle.net/10261/13118
DOI10.1016/j.micron.2008.01.022
ISSN0968-4328
Aparece en las colecciones: (IRN) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.