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Contribution of recombination and selection to molecular evolution of Citrus tristeza virus

AuthorsMartín, Susana ; Sambade, Adrián; Rubio, Luis; Vives, María C.; Moya, Patricia; Guerri, José; Elena, Santiago F. ; Moreno, Pedro
KeywordsVirus evolution
Molecular evolution
Genetic variability
Maximum likelihood
Molecular epidemiology
Selective constraints
Issue Date19-May-2009
PublisherSociety for General Microbiology
CitationJournal of General Virology 90: 1527-1538 (2009)
AbstractThe genetic variation of Citrus tristeza virus (CTV) was analyzed comparing the predominant sequence variants in seven genomic regions (p33, p65, p61, p18, p13, p20, and p23) of 18 pathogenically distinct isolates from seven different countries. Analyses of the selective constraints acting on each codon suggest that most regions were under purifying selection. Phylogenetic analysis show diverse patterns of molecular evolution for different genomic regions. A first clade composed by isolates genetically close to the reference mild isolates T385 or T30 was inferred from all genomic regions. A second clade, mostly comprising virulent isolates, was defined from regions p33, p65, p13, p20, and p23. For regions p65, p61, p18, p13, and p23 a third clade that mostly included South American isolates could not be related with any reference genotype. Phylogenetic relationships among isolates did not reflect their geographical origin, suggesting significant gene flow between geographically distant areas. Incongruent phylogenetic trees for different genomic regions suggested recombination events, an extreme that was supported by several recombination-detecting methods. A phylogenetic network incorporating the effect of recombination showed an explosive radiation pattern for the evolution of some isolates and grouped isolates by virulence. Taken together, the above results suggest that negative selection, gene flow, sequence recombination, and virulence may be important factors driving CTV evolution.
Description30 páginas, 3 figuras, 2 tablas.
Publisher version (URL)http://dx.doi.org/10.1099/vir.0.008193-0
Appears in Collections:(IBMCP) Artículos
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