English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/130944

Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine

AutorPuras, Gustavo; Martínez-Navarrete, Gema; Mashal, M.; Zarate, Jon; Agirre, Mireia; Ojeda, Edilberto; Grijalvo, Santiago; Eritja Casadellà, Ramón; Díaz-Tahoces, Ariadna; Avilés-Trigueros, Marcelino; Fernandez, E.; Pedraz, José Luís
Palabras claveRene therapy
Nonviral vectors
Fecha de publicación5-oct-2015
EditorAmerican Chemical Society
CitaciónMolecular Pharmaceutics 12(10) pp 3658–3671 (2015)
ResumenThe present study aimed to evaluate the incorporation of protamine into niosome/DNA vectors to analyze the potential application of this novel ternary formulation to deliver the pCMS-EGFP plasmid into the rat retina. Binary vectors based on niosome/DNA and ternary vectors based on protamine/DNA/niosomes were prepared and physicochemically characterized. In vitro experiments were performed in ARPE-19 cells. At 1:1:5 protamine/DNA/niosome mass ratio, the resulted ternary vectors had 150 nm size, positive charge, spherical morphology, and condensed, released, and protected the DNA against enzymatic digestion. The presence of protamine in the ternary vectors improved transfection efficiency, cell viability, and DNA condensation. After ocular administration, the EGFP expression was detected in different cell layers of the retina depending on the administration route without any sign of toxicity associated with the formulations. While subretinal administration transfected mainly photoreceptors and retinal pigment epithelial cells at the site of injection, intravitreal administration produced a more uniform distribution of the protein expression through the inner layers of the retina. The protein expression in the retina persisted for at least one month after both administrations. Our study highlights the flattering properties of protamine/DNA/niosome ternary vectors for efficient and safe gene delivery to the rat retina.
Versión del editorhttp://dx.doi.org/10.1021/acs.molpharmaceut.5b00422
Aparece en las colecciones: (IQAC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
MolPharm2015Protamine.pdf1,58 MBAdobe PDFVista previa
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.