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Título

Biocatalytic behaviour of immobilized Rhizopus oryzae lipase in the 1,3-selective ethanolysis of sunflower oil to obtain a biofuel similar to biodiesel

AutorLuna, Carlos; Verdugo, Cristóbal; Sancho, Enrique D.; Luna, Diego; Calero, Juan; Posadillo, Alejandro; Bautista, Felipa M.; Romero, Antonio A.
Palabras clavedemineralized sepiolite
biodiesel
immobilization
Rhizopus oryzae lipase
Biolipase-R
amorphous AlPO4/sepiolite
selective transesterification
monoglyceride
Ecodiesel
sunflower oil
Fecha de publicación4-ago-2014
EditorMolecular Diversity Preservation International
CitaciónMolecules 19: 11419-11439 (2014)
ResumenA new biofuel similar to biodiesel was obtained in the 1,3-selective transesterification reaction of sunflower oil with ethanol using as biocatalyst a Rhizopus oryzae lipase (ROL) immobilized on Sepiolite, an inorganic support. The studied lipase was a low cost powdered enzyme preparation, Biolipase-R, from Biocon-Spain, a multipurpose additive used in food industry. In this respect, it is developed a study to optimize the immobilization procedure of these lipases on Sepiolite. Covalent immobilization was achieved by the development of an inorganic-organic hybrid linker formed by a functionalized hydrocarbon chain with a pendant benzaldehyde, bonded to the AlPO4 support surface. Thus, the covalent immobilization of lipases on amorphous AlPO 4/sepiolite (20/80 wt %) support was evaluated by using two different linkers (p-hydroxybenzaldehyde and benzylamine-terephthalic aldehyde, respectively). Besides, the catalytic behavior of lipases after physical adsorption on the demineralized sepiolite was also evaluated. Obtained results indicated that covalent immobilization with the p-hydroxybenzaldehyde linker gave the best biocatalytic behavior. Thus, this covalently immobilized lipase showed a remarkable stability as well as an excellent capacity of reutilization (more than five successive reuses) without a significant loss of its initial catalytic activity. This could allow a more efficient fabrication of biodiesel minimizing the glycerol waste production. © 2014 by the authors.
Versión del editorhttp://dx.doi.org/10.3390/molecules190811419
URIhttp://hdl.handle.net/10261/130930
DOI10.3390/molecules190811419
Identificadoresissn: 1420-3049
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