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Title

Mesoporous silicas with tunable morphology for the immobilization of laccase

AuthorsGascón Pérez, Victoria ; Díaz Carretero, Isabel ; Márquez Álvarez, Carlos ; Blanco Martín, Rosa María
Keywordssupport
SBA-15
ordered mesoporous materials
MS-3030
laccase
biocatalysts
enzyme immobilization
functionalization
grafting
amorphous silica
Issue Date30-May-2014
PublisherMolecular Diversity Preservation International
CitationMolecules 19: 7057-7071 (2014)
AbstractSiliceous ordered mesoporous materials (OMM) are gaining interest as supports for enzyme immobilization due to their uniform pore size, large surface area, tunable pore network and the introduction of organic components to mesoporous structure. We used SBA-15 type silica materials, which exhibit a regular 2D hexagonal packing of cylindrical mesopores of uniform size, for non-covalent immobilization of laccase. Synthesis conditions were adjusted in order to obtain supports with different particle shape, where those with shorter channels had higher loading capacity. Despite the similar isoelectric points of silica and laccase and the close match between the size of laccase and the pore dimensions of these SBA-15 materials, immobilization was achieved with very low leaching. Surface modification of macro-/mesoporous amorphous silica by grafting of amine moieties was proved to significantly increase the isoelectric point of this support and improve the immobilization yield. © 2014 by the authors.
Publisher version (URL)http://dx.doi.org/10.3390/molecules19067057
URIhttp://hdl.handle.net/10261/130928
DOI10.3390/molecules19067057
Identifiersissn: 1420-3049
Appears in Collections:(ICP) Artículos
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