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Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB

AutorAlves, Joana S.; García-Galán, Cristina; Schein, Mirela F.; Silva, Alexandre M.; Barbosa, Oveimar; Ayub, Marco A. Z.; Fernández-Lafuente, Roberto ; Rodrigues, Rafael C.
Palabras claveesterification
butyl acetate
enzyme stability
enzyme reuse
ultrasound
lipase
Fecha de publicación7-jul-2014
EditorMolecular Diversity Preservation International
CitaciónMolecules 19(7): 9562-9576 (2014)
ResumenIt is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: Temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol: Acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system.
Versión del editorhttp://dx.doi.org/10.3390/molecules19079562
URIhttp://hdl.handle.net/10261/130914
DOI10.3390/molecules19079562
Identificadoresissn: 1420-3049
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