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Título

Sensitive and label-free detection of miRNA-145 by triplex formation

AutorAviñó, Anna; Huertas, César S.; Lechuga, Laura M. ; Eritja Casadellà, Ramón
Palabras clave8-Amino-2′-deoxyguanosine
Triplex
Parallel tail-clamp
SPR biosensor
Target miRNA-145
Fecha de publicación1-ene-2016
EditorSpringer
CitaciónAnalytical and Bioanalytical Chemistry 408(3): 885-893 (2016)
ResumenThe development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading sometimes to contradictory results. In this work, we study the use of modified parallel tail-clamps to detect a miRNA sequence involved in tumor suppression by triplex formation. Thermal denaturing curves and circular dichroism (CD) measurements have been performed to confirm that parallel clamps carrying 8-aminoguanine form the most stable triplex structures with their target miRNA. The modified tail-clamps have been tested as bioreceptors in a surface plasmon resonance (SPR) biosensor for the detection of miRNA-145. The detection limit was improved 2.4 times demonstrating that a stable triplex structure is formed between target miRNA and 8-aminoguanine tail-clamp bioreceptor. This new approach is an essential step toward the label-free and reliable detection of miRNA signatures for diagnostic purposes.
Versión del editorhttp://dx.doi.org/10.1007/s00216-015-9180-6
URIhttp://hdl.handle.net/10261/130888
DOI10.1007/s00216-015-9180-6
ISSN1618-2642
E-ISSN1618-2650
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