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Title

Sensitive and label-free detection of miRNA-145 by triplex formation

AuthorsAviñó, Anna; Huertas, César S.; Lechuga, Laura M. ; Eritja Casadellà, Ramón
Keywords8-Amino-2′-deoxyguanosine
Triplex
Parallel tail-clamp
SPR biosensor
Target miRNA-145
Issue Date1-Jan-2016
PublisherSpringer
CitationAnalytical and Bioanalytical Chemistry 408(3): 885-893 (2016)
AbstractThe development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading sometimes to contradictory results. In this work, we study the use of modified parallel tail-clamps to detect a miRNA sequence involved in tumor suppression by triplex formation. Thermal denaturing curves and circular dichroism (CD) measurements have been performed to confirm that parallel clamps carrying 8-aminoguanine form the most stable triplex structures with their target miRNA. The modified tail-clamps have been tested as bioreceptors in a surface plasmon resonance (SPR) biosensor for the detection of miRNA-145. The detection limit was improved 2.4 times demonstrating that a stable triplex structure is formed between target miRNA and 8-aminoguanine tail-clamp bioreceptor. This new approach is an essential step toward the label-free and reliable detection of miRNA signatures for diagnostic purposes.
Publisher version (URL)http://dx.doi.org/10.1007/s00216-015-9180-6
URIhttp://hdl.handle.net/10261/130888
DOI10.1007/s00216-015-9180-6
ISSN1618-2642
E-ISSN1618-2650
Appears in Collections:(IQAC) Artículos
(CIN2) Artículos
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