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Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor

AuthorsPinacho, Daniel G.; Sánchez Baeza, Francisco J.; Pividori, María Isabel; Marco, María Pilar CSIC ORCID
Keywordsfluoroquinolone antibiotics
food safety
antimicrobial residues
electrochemical immunosensor
magnetic beads
Issue Date28-Aug-2014
PublisherMolecular Diversity Preservation International
CitationSensors 14: 15965-15980 (2014)
AbstractAn amperometric magneto-immunosensor (AMIS) for the detection of residues of fluoroquinolone antibiotics in milk samples is described for the first time. The immunosensor presented combines magnetic beads biomodified with an antibody with a broad recognition profile of fluoroquinolones, a haptenized enzyme and a magnetic graphite-epoxy composite (m-GEC) electrode. After the immunochemical reaction with specific enzyme tracer, the antibody biomodified magnetic beads are easily captured by an electrode made of graphite-epoxy composite containing a magnet, which also acts as transducer for the electrochemical detection. In spite of the complexity of milk, the use of magnetic beads allows elimination of potential interferences caused by the matrix components; hence the AMIS could perform quantitative measurements, directly in these samples, without any additional sample cleanup or extraction step. The immunosensor is able to detect up to seven different fluoroquinolones far below the MRLs defined by the UE for milk; for example ciprofloxacin is detected directly in milk with an IC50 of 0.74 μg/L and a LOD of 0.009 μg/L. This strategy offers great promise for rapid, simple, cost-effective, and on-site analysis fluoroquinolones in complex samples. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
Publisher version (URL)http://dx.doi.org/10.3390/s140915965
Identifiersissn: 1424-8220
Appears in Collections:(IQAC) Artículos
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