English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/130492
Título

Activation of nuclear receptor NR5A2 increases Glut4 expression and glucose metabolism in muscle cells

AutorBolado-Carrancio, A.; Riancho, José A.; Sainz, Jesús ; Rodríguez-Rey, José C.
Palabras claveC2C12
Glucose metabolism
Muscle
GLUT4
NR5A2
Fecha de publicación2014
EditorElsevier
CitaciónBiochemical and Biophysical Research Communications 446(2): 614-619 (2014)
ResumenNR5A2 is a nuclear receptor which regulates the expression of genes involved in cholesterol metabolism, pluripotency maintenance and cell differentiation. It has been recently shown that DLPC, a NR5A2 ligand, prevents liver steatosis and improves insulin sensitivity in mouse models of insulin resistance, an effect that has been associated with changes in glucose and fatty acids metabolism in liver. Because skeletal muscle is a major tissue in clearing glucose from blood, we studied the effect of the activation of NR5A2 on muscle metabolism by using cultures of C2C12, a mouse-derived cell line widely used as a model of skeletal muscle. Treatment of C2C12 with DLPC resulted in increased levels of expression of GLUT4 and also of several genes related to glycolysis and glycogen metabolism. These changes were accompanied by an increased glucose uptake. In addition, the activation of NR5A2 produced a reduction in the oxidation of fatty acids, an effect which disappeared in low-glucose conditions. Our results suggest that NR5A2, mostly by enhancing glucose uptake, switches muscle cells into a state of glucose preference. The increased use of glucose by muscle might constitute another mechanism by which NR5A2 improves blood glucose levels and restores insulin sensitivity.
URIhttp://hdl.handle.net/10261/130492
DOI10.1016/j.bbrc.2014.03.010
Identificadoresdoi: 10.1016/j.bbrc.2014.03.010
e-issn: 1090-2104
issn: 0006-291X
Aparece en las colecciones: (IBBTEC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.