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| Título: | Complete proteome of a quinolone-resistant Salmonella Typhimurium phage type DT104B clinical strain |
Autor: | Correia, Susana; Toro, María de CSIC ORCID CVN; Igrejas, Gilberto | Palabras clave: | Aminoglycoside 6'-N-acetyltransferase type Ib-cr4 Salmonella enterica serovar Typhimurium DT104B SL1344 Proteome |
Fecha de publicación: | 2014 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | International Journal of Molecular Sciences 15(8): 14191-14219 (2014) | Resumen: | Salmonellosis is one of the most common and widely distributed foodborne diseases. The emergence of Salmonella strains that are resistant to a variety of antimicrobials is a serious global public health concern. Salmonella enterica serovar Typhimurium definitive phage type 104 (DT104) is one of these emerging epidemic multidrug resistant strains. Here we collate information from the diverse and comprehensive range of experiments on Salmonella proteomes that have been published. We then present a new study of the proteome of the quinolone-resistant Se20 strain (phage type DT104B), recovered after ciprofloxacin treatment and compared it to the proteome of reference strain SL1344. A total of 186 and 219 protein spots were recovered from Se20 and SL1344 protein extracts, respectively, after two-dimensional gel electrophoresis. The signatures of 94% of the protein spots were successfully identified through matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). Three antimicrobial resistance related proteins, whose genes were previously detected by polymerase chain reaction (PCR), were identified in the clinical strain. The presence of these proteins, dihydropteroate synthase type-2 (sul2 gene), aminoglycoside resistance protein A (strA gene) and aminoglycoside 6'-N-acetyltransferase type Ib-cr4 (aac(6')-Ib-cr4 gene), was confirmed in the DT104B clinical strain. The aac(6')-Ib-cr4 gene is responsible for plasmid-mediated aminoglycoside and quinolone resistance. This is a preliminary analysis of the proteome of these two S. Typhimurium strains and further work is being developed to better understand how antimicrobial resistance is developing in this pathogen. | Descripción: | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license.-- et al. | Versión del editor: | http://dx.doi.org/10.3390/ijms150814191 | URI: | http://hdl.handle.net/10261/130489 | DOI: | 10.3390/ijms150814191 | Identificadores: | doi: 10.3390/ijms150814191 e-issn: 1422-0067 issn: 1661-6596 |
| Aparece en las colecciones: | (IBBTEC) Artículos |
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| SalmonellaTyphimurium.pdf | 1,75 MB | Adobe PDF |  Visualizar/Abrir |
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