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Title

Eutylenchus excretorius Ebsary Eveleigh, 1981 (Nematoda: Tylodorinae) from Spain with approaches to molecular phylogeny of related genera

AuthorsPalomares Rius, Juan E. ; Subbotin, Sergei A.; Liébanas, Gracia; Landa, Blanca B. ; Castillo, Pablo
Issue Date2009
PublisherBrill Academic Publishers
CitationNematology 11,343-354.
AbstractNematode surveys in indigenous vegetation in northern Spain revealed the presence of a nematode population of the genus Eutylenchus associated with moist sandy soils in the rhizosphere of common reed (Phragmites sp.) on the banks of the Tera river in Garray (Soria province). Morphological and morphometrical studies on this population fits with Eutylenchus excretorius, representing the first report for Spain and southern Europe and the fifth report in Europe after Germany, Poland, Czech Republic and Russia. SEM studies were carried out for the first time on this species and showed four lips separated by deep grooves. Each lip bears an elongated, flexible, recurved projection (seta) 12 (11-13) μm long, proximal third wide, gradually attenuating, distal end rounded. Molecular characterisation of E. excretorius using several genes is provided. The sequence of D2-D3 expansion segments of 28S rRNA gene of this population was identical to a previously studied sample from Germany. Phylogenetic analysis using D2-D3 of 28S rRNA and partial 18S rRNA gene sequences of tylenchid nematodes revealed that E. excretorius clustered with moderate support with Cephalenchus hexalineatus. The position of E. excretorius on majority consensus Bayesian phylogenetic tree reconstructed using heat shock protein 90 gene sequence was not well resolved.
URIhttp://hdl.handle.net/10261/13027
ISBN1568-5411
Appears in Collections:(IAS) Artículos
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