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Requirement of different Cv-c/DLC RhoGAP protein domains for epithelial morphogenesis

AuthorsAguilar, Mario ; Sotillos, Sol ; Foglia, Filippo ; Castelli-Gair Hombría, James
Issue Date2013
Citation23rd European Drosophila Research Congress (2013)
AbstractCrossveinless-c (Cv-c) is a RhoGAP protein required for Rho1 mediated actin reorganization in the Drosophila embryo. In the ectoderm, transcriptional activation of cv-c is required for the normal invagination of the tracheal pits, the salivary glands and the posterior spiracles. Cv-cRhoGAP localizes to the basolateral membrane in a complementary pattern to the apically localizing RhoGEFs proteins. As a result of the subcellular localization of its positive and negative regulators, Rho1GTP accumulates apically where induces the formation of a contractile acto-myosin cytoskeleton. To understand how Cv-c functions we dissected the protein to find out the domains required for its subcellular localization and enzymatic activity. Cv-cRhoGAP has three conserved domains: a SAM protein-protein interaction domain, a GAP RhoGTPase interacting domain and a START putative lipid binding domain, as well as a long non-conserved region. We show that the GAP and START domains are required for Cv-c activity while the non-conserved region mediates the subcellular membrane localization. The same domain architecture is also present in the human DLC-1 (Deleted in Liver Cancer-1), DLC-2 and DLC-3 tumour suppressor proteins. Expression of DLC-1 in Drosophila is inactive but if fused to the Cv-c non-conserved domain causes the same effects as Cv-c. Expression of unmodified DLC-3, which localizes to the membrane in vertebrate cell cultures, causes in Drosophila weak phenotypes similar to those of Cv-c. Thus, the function of Cv-c/DLC RhoGAP proteins is conserved suggesting Drosophila as a model to study DLC function.
DescriptionResumen del póster presentado al 23rd European Drosophila Research Congress, celebrado en Barcelona (España) del 16 al 19 de octubre de 2013.
Appears in Collections:(CABD) Comunicaciones congresos
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