English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/130187
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Dynamics of nuclear compartments in C. elegans

AuthorsSharma, Rahul; Gómez-Saldívar, Georgina ; Kind, Jop; Steensel, Bas van; Askjaer, Peter ; Meister, Peter
Issue Date2013
Citation19th International C. elegans Meeting (2013)
AbstractDuring development and differentiation, the genome gets progressively organized inside the nuclear space. The function of genome nuclear organization remains elusive, although a number of human diseases are linked to mutations in structural elements of the nucleus. Decreasing mobility of chromatin and chromatin factors moreover correlates with loss of differentiation potential, suggesting chromatin plasticity could be an integral part of pluripotency. Similarly to mammalian cells, the worm genome gets partitioned during cell fate acquisition into active and silent domains inside the nuclear space. In C. elegans current data suggest that the nuclear interior is a rather active domain, while the nuclear periphery is a mosaic of active and silent compartments. The dynamics of these domains during physiological challenges, cell fate determination or during organismal aging is however poorly understood. Genomic characterization of subnuclear domains has been achieved using damID, a technique in which a fusion protein between the E. coli adenine methyltransferase dam is fused to a nuclear protein specific of the nuclear compartment of interest. Adenines in genomic stretches proximal to this protein are methylated and this can be detected using microarrays or sequencing. damID has been successfully used to uncover DAF-16 target genes, but also the nuclear organization of chromosomes relative to the nuclear lamina using a dam fusion to LMN-1, the unique worm lamin. Using a newly developed methylated adenine binder, we are now able to track in vivo nuclear compartments in developing and aging worms. We will present this system in the worm as well as the first results on dynamics of subnuclear compartments in aging or physiologically challenged worms.
DescriptionResumen del póster presentado al 19th International C. elegans Meeting, celebrado en Los Angeles, California (US) del 26 al 30 de junio de 2013.
Appears in Collections:(CABD) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.