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Identification of the LMN-1 and EMR-1 DNA binding sites reveal the genome organization at the nuclear envelope in C. elegans

AutorGonzález-Aguilera, Cristina ; Askjaer, Peter
Fecha de publicación2012
Citación22nd IUBMB and 37th FEBS (2012)
ResumenBesides its role as a barrier between the nucleus and the cytoplasm, in the last years it has been revealed as a dynamic structure that plays an important role in the regulation of gene expression and chromatin organization. Traditionally, the NE has been associated with heterochromatin and silent DNA. However, recent studies have shown that there is also active chromatin at the nuclear periphery and genes that change their localization depending on their transcriptional state. Despite the efforts realized, the molecular mechanisms underlining all these processes are not well understood. Here we have developed tools to perform genome wide analysis using the DamID and RNA-seq methods. DamID is based on the expression in vivo of chimeric proteins containing an adenine methyltransferase (Dam) from E. coli that methylates the DNA in the vicinity of native binding sites of a chromatin-interacting protein. We have created Caenorhabditis elegans strains containing single copy insertions of Dam fused to two NE proteins, emerin/EMR-1 and lamin/LMN-1. Employing a genetically amenable model system enables us to analyze nuclear architecture across several NE mutant backgrounds, and trough different developmental stages. Besides, we have performed RNA-seq analysis in wild type and NE mutants to correlate the position of genes with their transcriptional state.
DescripciónResumen del póster presentado al 22nd IUBMB & 37th FEBS Congress, celebrado en Sevilla (España) del 4 al 9 de septiembre de 2012.
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