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Metabolic changes induced by resveratrol in tumoral cells

AuthorsSanta-Cruz Calvo, Sara; Pane Rebollo, Valentina; Navas, Plácido ; López-Lluch, Guillermo
Issue Date2012
Citation22nd IUBMB and 37th FEBS (2012)
AbstractResveratrol (RSV) is a stilbenoid known to activate sirtuins and to show prolongevity effects (Baur et al., 2006). It is known that the treatment of tumor cell lines with RSV produces a dosedependent S-phase delay starting as early as 6-12 hours of incubation. Cell cycle distribution seems to return to normal values after 48 hour of treatment depending on the dose of RSV. After this delay, metabolism of tumoral cells has passed from glycolitic to more respiratory metabolism. We have previously shown that RSV increases mitochondrial mass in several cell types including tumoral cells such as the human epithelial cervical cancer cell line HeLa (Baur et al., 2006). RSV increased oxygen consumption in glycolitic HeLa cells accompanied by the modification of the activity and protein levels of electron transport chain components. Further, RSV induced a significant increase in b-oxidation. This effect was accompanied by increases in b-oxidation-related protein levels such as CPT-1 and CPT-2. Further, RSV also modified levels of pyridine nucleotides in cells by decreasing the ratio NADH/NAD+. Although RSV is considered an antioxidant, treatment of HeLa cells with this compound increases ROS levels in a time and dose-dependent mechanism. This increase in ROS levels was produced in parallel of changed found in mitochondria. All this process was accompanied by the induction of autophagic mechanisms as determined by the increase in autophagy protein levels such as Beclin-1 or ATG-3. Our results indicate that RSV affects cell cycle progression at the same time that it is modifying the metabolism of tumoral cells from glycolysis to respiration. In this process, mitochondrial remodeling by induction of biogenesis at the same time than autophagy is involved. Baur et al. Nature 2007;444(7117):337-342.
DescriptionResumen del póster presentado al 22nd IUBMB & 37th FEBS Congress, celebrado en Sevilla (España) del 4 al 9 de septiembre de 2012.
Appears in Collections:(CABD) Comunicaciones congresos
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