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Counter-repression: an alternative strategy for STAT's transcriptional activation

AutorPinto, Pedro ; Espinosa-Vázquez, José Manuel; Castelli-Gair Hombría, James
Fecha de publicación2012
Citación10th EMBL Conference (2012)
ResumenThe JAWSTAT signalling pathway is highly conserved among vertebrates and invertebrates where it is not only required for key developmental processes, but also for homeostasis, with misregulation of the pathway associated with the development of several types of cancer. For this reason, identification of STAT downstream targets has been of the utmost importance. Despite this, the actual molecular mechanism of STAT in the activation of its targets remains unclear. Drosophila, with a simple JAWSTAT pathway compared to vertebrates, provides a good model to address how STAT is able to regulate gene expression. Although several STAT-activated enhancers have been previously described, little is known about the function of STAT in the activation of these cis-regulatory elements. Previous work showed that, in Drosophila, JAWSTAT is required for posterior spiracle morphogenesis, where it directs the transcription of specific target genes. One of these targets is the cell polarity gene crumbs (crb) that is up-regulated in the posterior spiracle primordia through the direct activation by JAWSTAT of an enhancer localized in an intronic region. The activation of this enhancer exclusively in the posterior spiracles and not in other cells where the pathway is active, provides a model to study gene specific activation by STAT. We have dissected the enhancer and analyzed it genetically and biochemically. We will present evidence suggesting that despite STAT being required for the activity of the spiracle enhancer in its normal genomic context, STAT does not act as a direct transcriptional activator. Instead, we show that other factors provide the spatial specific activation of the crb enhancer with STAT, rather than directly activating the enhancer, being required to counteract the activity of a repressor. To our knowledge, this is the first time that such a function has been proposed for STAT proteins.
DescripciónResumen del póster presentado a la 10th EMBL Conference: "Transcription and Chromatin", celebrada en Heidelberg (Alemania) del 25 al 28 de agosto de 2012.
Aparece en las colecciones: (CABD) Comunicaciones congresos
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