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dc.contributor.authorGaspar, Helenaes_ES
dc.contributor.authorFerreira, R.es_ES
dc.contributor.authorGonzález Grau, Juan Migueles_ES
dc.contributor.authorClara, Maria I. E.es_ES
dc.contributor.authorSantana, Margaridaes_ES
dc.identifier.citationCurrent Microbiology 72(4):370-376 (2016)es_ES
dc.description7 páginas.-- 3 figuras.-- 3 tablas.-- 30 referenciases_ES
dc.description.abstractβ-Proteobacteria is one of the most abundant phylum in soils, including autotrophic and heterotrophic ammonium-consumers with relevance in N circulation in soils. The effects of high-temperature events and phytosanitary treatments, such as copper amendments, on soil bacterial communities relevant to N-cycling remain to be studied. As an example, South Portugal soils are seasonally exposed to high-temperature periods, the temperature at the upper soil layers can reach over 40 °C. Here, we evaluated the dynamics of mesophilic and thermophilic bacteria from a temperate soil, in particular of heterotrophic β-Proteobacteria, regarding the ammonium equilibrium, as a function of temperature and copper treatment. Soil samples were collected from an olive orchard in southern Portugal. Selective enrichments were performed from samples under different conditions of temperature (30 and 50 °C) and copper supplementation (100 and 500 µM) in order to mime seasonal variations and phytosanitary treatments. Changes in the microbial communities under these conditions were examined by denaturing gradient gel electrophoresis, a molecular fingerprint technique. At moderate temperature—30 °C—either without or with copper addition, dominant members were identified as different strains belonging to genus Massilia, a genus of the Oxalobacteraceae (β-Proteobacteria), while at 50 °C, members of the Brevibacillus genus, phylum Firmicutes were also represented. Ammonium production during bacterial growth at moderate and high temperatures was not affected by copper addition. Results indicate that both copper and temperature selected specific tolerant bacterial strains with consequences for N-cycling in copper-treated orchards.es_ES
dc.description.sponsorshipThis work was funded by FEDER Funds through the Operational Programme for Competitiveness Factors COMPETE and National Funds through FCT Foundation for Science and Technology under the Strategic Project PEst-C/AGR/UI0115/2011. We thank Eng. G. Pinheiro, from Eugénio Almeida Foundation (FEA) for providing access to the FEA olive orchard.es_ES
dc.titleInfluence of Temperature and Copper on Oxalobacteraceae in Soil Enrichmentses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderEuropean Commissiones_ES
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