English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/129945
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

How soft is a single protein?: Stress-strain curve of antibody pentamers with 5 pN and 50 pm resolutions

AutorPerrino, Alma P. ; García García, Ricardo
Fecha de publicación2016
EditorRoyal Society of Chemistry (Great Britain)
CitaciónNanoscale 8: 9151-9158 (2016)
ResumenUnderstanding the mechanical functionalities of complex biological systems requires the measurement of the mechanical compliance of their smallest components. Here, we develop a force microscopy method to quantify the softness of a single antibody pentamer by measuring the stress–strain curve with force and deformation resolutions, respectively, of 5 pN and 50 pm. The curve shows three distinctive regions. For ultrasmall compressive forces (5–75 pN), the protein's central region shows that the strain and stress are proportional (elastic regime). This region has an average Young's modulus of 2.5 MPa. For forces between 80 and 220 pN, the stress is roughly proportional to the strain with a Young's modulus of 9 MPa. Higher forces lead to irreversible deformations (plastic regime). Full elastic recovery could reach deformations amounting to 40% of the protein height. The existence of two different elastic regions is explained in terms of the structure of the antibody central region. The stress–strain curve explains the capability of the antibody to sustain multiple collisions without any loss of biological functionality.
Versión del editorhttp://dx.doi.org/10.1039/c5nr07957h
URIhttp://hdl.handle.net/10261/129945
DOI10.1039/c5nr07957h
ISSN2040-3364
E-ISSN2040-3372
Aparece en las colecciones: (ICMM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
ProteinStress-strainCurve_NanoScale_Perrino.pdf890,42 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.