English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/129856
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Gold-coated superparamagnetic nanoparticles for single methyl discrimination in DNA aptamers

AuthorsTintoré, María; Mazzini, Stefania; Polito, Laura; Marelli, Marcello; Latorre, Alfonso; Somoza, Álvaro; Aviñó, Anna; Fàbrega, Carme; Eritja Casadellà, Ramón
KeywordsAptamers
Biosensor
Gold-coated superparamagnetic nanoparticles
Nanotechnology
Issue Date18-Nov-2015
PublisherMultidisciplinary Digital Publishing Institute
CitationInternational Journal of Molecular Sciences
AbstractAu- and iron-based magnetic nanoparticles (NPs) are promising NPs for biomedical applications due to their unique properties. The combination of a gold coating over a magnetic core puts together the benefits from adding the magnetic properties to the robust chemistry provided by the thiol functionalization of gold. Here, the use of Au-coated magnetic NPs for molecular detection of a single methylation in DNA aptamer is described. Binding of α-thrombin to two aptamers conjugated to these NPs causes aggregation, a phenomenon that can be observed by UV, DLS and MRI. These techniques discriminate a single methylation in one of the aptamers, preventing aggregation due to the inability of α-thrombin to recognize it. A parallel study with gold and ferromagnetic NPs is detailed, concluding that the Au coating of FexOy NP does not affect their performance and that they are suitable as complex biosensors. These results prove the high detection potency of Au-coated SPIONs for biomedical applications especially for DNA repair detection.
Publisher version (URL)http://www.mdpi.com/1422-0067/16/11/26046
URIhttp://hdl.handle.net/10261/129856
DOI10.3390/ijms161126046
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
ijms-16-26046TBAgold.pdf1,72 MBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.