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dc.contributor.authorMarrero, Patriciaen_US
dc.contributor.authorPérez Padilla, Daviden_US
dc.contributor.authorValdés, Franciscoen_US
dc.contributor.authorNogales, Manuel-
dc.date.accessioned2009-05-12T06:18:13Z-
dc.date.available2009-05-12T06:18:13Z-
dc.date.issued2007-01-12en_US
dc.identifier.citationChemoecology 17(1): 47-50 (2007)en_US
dc.identifier.issn0937-7409-
dc.identifier.urihttp://hdl.handle.net/10261/12983-
dc.description4 pages, 1 table.-- Printed version published Mar 2007.-
dc.description.abstractThree chemical viability tests were evaluated in the seed dispersal system of Rubia fruticosa, in which three main groups of dispersers participate: reptiles, birds and mammals. Tetrazolium chloride (TTC) and indigo carmine (IC) indicated a lower viability of seeds from droppings of introduced rabbits (Oryctolagus cuniculus) than of those from control plants and the native dispersers, lizards and gulls. In the rabbit seed treatment, significant differences were observed between results obtained with TTC and IC tests. Interpretation of these data, due to the presence of doubtful embryo staining, was more difficult using the IC test. Furthermore, some seeds that were clearly dead had been underestimated. In contrast with results obtained from the two staining methods, the EC test did not confirm that viability of control seeds and those seeds consumed by native dispersers were clearly higher than in seeds ingested by O. cuniculus. Further, compared to the other two tests, the EC method requires more careful handling of the embryo during the extraction process to avoid errors in viability estimation, since this method measures concentration of electrolytes that are released through cellular membranes. Thus, TTC was the most reliable test to assess seed viability in the seed dispersal system of R. fruticosa, and these results agree with those obtained in previous germination experiments made on the same set of seeds given the same treatments.-
dc.description.abstractThree chemical viability tests were evaluated in the seed dispersal system of Rubia fruticosa, in which three main groups of dispersers participate: reptiles, birds and mammals. Tetrazolium chloride (TTC) and indigo carmine (IC) indicated a lower viability of seeds from droppings of introduced rabbits (Oryctolagus cuniculus) than of those from control plants and the native dispersers, lizards and gulls. In the rabbit seed treatment, significant differences were observed between results obtained with TTC and IC tests. Interpretation of these data, due to the presence of doubtful embryo staining, was more difficult using the IC test. Furthermore, some seeds that were clearly dead had been underestimated. In contrast with results obtained from the two staining methods, the EC test did not confirm that viability of control seeds and those seeds consumed by native dispersers were clearly higher than in seeds ingested by O. cuniculus. Further, compared to the other two tests, the EC method requires more careful handling of the embryo during the extraction process to avoid errors in viability estimation, since this method measures concentration of electrolytes that are released through cellular membranes. Thus, TTC was the most reliable test to assess seed viability in the seed dispersal system of R. fruticosa, and these results agree with those obtained in previous germination experiments made on the same set of seeds given the same treatments.-
dc.description.sponsorshipThis research was supported by grants to Patricia Marrero and David P. Padilla from the Proyectos Intramurales Especiales, 2004 3 0E 169 of the Spanish National Scientific Research Council (Consejo Superior de Investigaciones Científicas) and the Canarian Government, respectively. It was partially financed by the projects BOS2001-0610 (Ministerio de Educación y Ciencia) and PI042004/037 (Consejería de Educación, Cultura y Deportes, Gobierno de Canarias), which are partially supported by Feder funds from the European Union.-
dc.format.extent2373 bytes-
dc.format.extent143435 bytes-
dc.format.mimetypetext/plain-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherSpringer-
dc.rightsclosedAccessen_US
dc.subjectTetrazolium-
dc.subjectIndigo carmine-
dc.subjectElectroconductivity-
dc.subjectMacaronesian Islands-
dc.subjectRubia fruticosa-
dc.subjectVertebrate dispersers-
dc.titleComparison of three chemical tests to assess seed viability: the seed dispersal system of the Macaronesian endemic plant Rubia fruticosa (Rubiaceae) as an exampleen_US
dc.typeArtículoen_US
dc.identifier.doi10.1007/s00049-006-0360-x-
dc.relation.publisherversionhttp://dx.doi.org/10.1007/s00049-006-0360-x-
dc.identifier.e-issn1423-0445-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderGobierno de Canarias-
dc.contributor.funderMinisterio de Educación y Ciencia (España)-
dc.contributor.funderEuropean Commission-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
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