Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/129819
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Synthesis of oligonucleotides carrying fluorescently labelled O6-alkylguanine for measuring hAGT activity |
Autor: | Tintoré, María CSIC; Eritja Casadellà, Ramón CSIC ORCID ; Grijalvo, Santiago CSIC ORCID; Fàbrega, Carme CSIC ORCID | Palabras clave: | DNA repair Fluorescence Mitsunobu O6-alkylguanine-DNA alkyltransferase Oligonucleotide synthesis |
Fecha de publicación: | 15-nov-2015 | Editor: | Elsevier | Citación: | Bioorganic and Medicinal Chemistry Letters | Resumen: | O6-alkylguanine-DNA-alkyltransferase (hAGT) activity provides resistance to cancer chemotherapeutic agents and its inhibition enhances chemotherapy. We herein present the development of a novel fluorescence assay for the detection of hAGT activity. We designed a dsDNA sequence containing a fluorophore-quencher pair, where the fluorophore was attached to an O6-benzylguanine. This precursor was synthesized using the Mitsunobu reaction to introduce the benzyl group. The alkyl-fluorophore group is transferred to the active site during the dealkylation, producing an increase in fluorescence which is correlated to hAGT activity. This assay can be used for the evaluation of potential inhibitors of hAGT in a straightforward manner. | Versión del editor: | http://www.sciencedirect.com/science/article/pii/S0960894X15300998 | URI: | http://hdl.handle.net/10261/129819 | DOI: | 10.1016/j.bmcl.2015.09.065 |
Aparece en las colecciones: | (IQAC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
BCML2015.pdf | 653,51 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
6
checked on 16-mar-2024
WEB OF SCIENCETM
Citations
4
checked on 27-feb-2024
Page view(s)
193
checked on 19-mar-2024
Download(s)
282
checked on 19-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.