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Atomic Force Microscopy and Voltammetric Investigation of Quadruplex Formation between a Triazole-Acridine Conjugate and Guanine-Containing Repeat DNA Sequences

AutorChiorcea-Paquim, Ana Maria; Pontinha, Ana Dora Rodrigues Rodrigues; Eritja Casadellà, Ramón; Lucarelli, Genny; Sparapani, Silvia; Neidle, Stephen; Oliveira-Brett, Ana Maria
Palabras claveQuadruplex formation
Voltammetric investigation
chemical structure
Fecha de publicación16-jun-2015
EditorAmerican Chemical Society
CitaciónAnalytical Chemistry
ResumenThe interactions of the Tetrahymena telomeric repeat sequence d(TG4T) and the polyguanylic acid (poly(G)) sequence with the quadruplex-targeting triazole-linked acridine ligand GL15 were investigated using atomic force microscopy (AFM) at a highly oriented pyrolytic graphite and voltammetry at a glassy carbon electrode. GL15 interacted with both sequences, in a time dependent manner, and G-quadruplex formation was detected. AFM showed the adsorption of quadruplexes as small d(TG4T) and poly(G) spherical aggregates and large quadruplex-based poly(G) assemblies, and voltammetry showed the decrease and disappearance of GL15 and guanine oxidation peak currents and appearance of the G-quadruplex oxidation peak. The GL15 molecule strongly stabilized and accelerated G-quadruplex formation in both Na+ and K+ ion-containing solution, although only K+ promoted the formation of perfectly aligned tetra-molecular G-quadruplexes. The small-molecule complex with the d(TG4T) quadruplex is discrete and approximately globular, whereas the G-quadruplex complex with poly(G) is formed at a number of points along the length of the polynucleotide, analogous to beads on a string.
Versión del editorhttp://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b00743
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