English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/129818
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Atomic Force Microscopy and Voltammetric Investigation of Quadruplex Formation between a Triazole-Acridine Conjugate and Guanine-Containing Repeat DNA Sequences

AuthorsChiorcea-Paquim, Ana Maria; Pontinha, Ana Dora Rodrigues Rodrigues; Eritja Casadellà, Ramón ; Lucarelli, Genny; Sparapani, Silvia; Neidle, Stephen; Oliveira-Brett, Ana Maria
KeywordsQuadruplex formation
Carbon
Electrodes
Voltammetric investigation
DNA
chemical structure
Triazoles
Issue Date16-Jun-2015
PublisherAmerican Chemical Society
CitationAnalytical Chemistry
AbstractThe interactions of the Tetrahymena telomeric repeat sequence d(TG4T) and the polyguanylic acid (poly(G)) sequence with the quadruplex-targeting triazole-linked acridine ligand GL15 were investigated using atomic force microscopy (AFM) at a highly oriented pyrolytic graphite and voltammetry at a glassy carbon electrode. GL15 interacted with both sequences, in a time dependent manner, and G-quadruplex formation was detected. AFM showed the adsorption of quadruplexes as small d(TG4T) and poly(G) spherical aggregates and large quadruplex-based poly(G) assemblies, and voltammetry showed the decrease and disappearance of GL15 and guanine oxidation peak currents and appearance of the G-quadruplex oxidation peak. The GL15 molecule strongly stabilized and accelerated G-quadruplex formation in both Na+ and K+ ion-containing solution, although only K+ promoted the formation of perfectly aligned tetra-molecular G-quadruplexes. The small-molecule complex with the d(TG4T) quadruplex is discrete and approximately globular, whereas the G-quadruplex complex with poly(G) is formed at a number of points along the length of the polynucleotide, analogous to beads on a string.
Publisher version (URL)http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b00743
URIhttp://hdl.handle.net/10261/129818
DOI10.1021/acs.analchem.5b00743
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
AnalChem2015.pdf1,85 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.