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Title

An RNA polymerase III subunit determines sites of retrotransposon integration

AuthorsFlores, Amando ; Lesage, Pascale
Issue Date2015
PublisherAmerican Association for the Advancement of Science
CitationScience 348(6234): 585-588 (2015)
AbstractMobile genetic elements are ubiquitous. Their integration site influences genome stability and gene expression. The Ty1 retrotransposon of the yeast Saccharomyces cerevisiae integrates upstream of RNA polymerase III (Pol III)-transcribed genes, yet the primary determinant of target specificity has remained elusive. Here we describe an interaction between Ty1 integrase and the AC40 subunit of Pol III and demonstrate that AC40 is the predominant determinant targeting Ty1 integration upstream of Pol III-transcribed genes. Lack of an integrase-AC40 interaction dramatically alters target site choice, leading to a redistribution of Ty1 insertions in the genome, mainly to chromosome ends. The mechanism of target specificity allows Ty1 to proliferate and yet minimizes genetic damage to its host.
URIhttp://hdl.handle.net/10261/129519
DOI10.1126/science.1259114
Identifiersdoi: 10.1126/science.1259114
issn: 0036-8075
e-issn: 1095-9203
Appears in Collections:(CABD) Artículos
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