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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Ferrer-Mayorga, Gemma | es_ES |
dc.contributor.author | Álvarez-Díaz, S. | es_ES |
dc.contributor.author | Valle, Noelia | es_ES |
dc.contributor.author | De Las Rivas, Javier | es_ES |
dc.contributor.author | Mendes, Marta | es_ES |
dc.contributor.author | Barderas, Rodrigo | es_ES |
dc.contributor.author | Casal, J. Ignacio | es_ES |
dc.contributor.author | Lafarga, Miguel | es_ES |
dc.contributor.author | Muñoz Terol, Alberto | es_ES |
dc.date.accessioned | 2016-02-26T09:36:44Z | - |
dc.date.available | 2016-02-26T09:36:44Z | - |
dc.date.issued | 2015-10-30 | - |
dc.identifier.citation | Journal of Biological Chemistry 290(44): 26533-26548 (2015) | es_ES |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10261/129490 | - |
dc.description | Ferrer-Mayorga, Gemma et al. | es_ES |
dc.description.abstract | Cystatin D is an inhibitor of lysosomal and secreted cysteine proteases. Strikingly, cystatin D has been found to inhibit proliferation, migration, and invasion of colon carcinoma cells indicating tumor suppressor activity that is unrelated to protease inhibition. Here, we demonstrate that a proportion of cystatin D locates within the cell nucleus at specific transcriptionally active chromatin sites. Consistently, transcriptomic analysis show that cystatin D alters gene expression, including that of genes encoding transcription factors such as RUNX1, RUNX2, and MEF2C in HCT116 cells. In concordance with transcriptomic data, quantitative proteomic analysis identified 292 proteins differentially expressed in cystatin D-expressing cells involved in cell adhesion, cytoskeleton, and RNA synthesis and processing. Furthermore, using cytokine arrays we found that cystatin D reduces the secretion of several protumor cytokines such as fibroblast growth factor-4, CX3CL1/fractalkine, neurotrophin 4 oncostatin-M, pulmonary and activation-regulated chemokine/CCL18, and transforming growth factor B3. These results support an unanticipated role of cystatin D in the cell nucleus, controlling the transcription of specific genes involved in crucial cellular functions, which may mediate its protective action in colon cancer. | es_ES |
dc.description.sponsorship | This work was supported by Spanish Ministry of Economy and Competitiveness-Fondo Europeo de Desarrollo Regional (FEDER) Grant SAF2013-43468-R, Comunidad de Madrid Grant Colomics2 S2010/BMD-2344, and FEDER-Instituto de Salud Carlos III Grant RD12/0036/0021. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Biochemistry and Molecular Biology | es_ES |
dc.relation | MINECO/ICTI2013-2016/SAF2013-43468-R | - |
dc.relation | S2010/BMD-2344/Colomics2 | es_ES |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | es_ES |
dc.subject | Colon cancer | es_ES |
dc.subject | Cysteine proteases | es_ES |
dc.subject | Cytokine | es_ES |
dc.subject | Gene expression | es_ES |
dc.subject | Protease inhibitor | es_ES |
dc.title | Cystatin D locates in the nucleus at sites of active transcription and modulates gene and protein expression | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1074/jbc.M115.660175 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1074/jbc.M115.660175 | es_ES |
dc.identifier.e-issn | 1083-351X | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | Comunidad de Madrid | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.relation.csic | Sí | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.pmid | 26364852 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
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