English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/129463
Título

Chromatids segregate without centrosomes during Caenorhabditis elegans mitosis in a Ran- and CLASP-dependent manner

AutorNahaboo, Wallis; Zouak, Melissa; Askjaer, Peter ; Delattre, Marie
Fecha de publicación2015
EditorAmerican Society for Cell Biology
CitaciónMolecular Biology of the Cell 26(11): 2020-2029 (2015)
ResumenDuring mitosis, chromosomes are connected to a microtubule-based spindle. Current models propose that displacement of the spindle poles and/or the activity of kinetochore microtubules generate mechanical forces that segregate sister chromatids. Using laser destruction of the centrosomes during Caenorhabditis elegans mitosis, we show that neither of these mechanisms is necessary to achieve proper chromatid segregation. Our results strongly suggest that an outward force generated by the spindle midzone, independently of centrosomes, is sufficient to segregate chromosomes in mitotic cells. Using mutant and RNAi analysis, we show that the microtubule-bundling protein SPD-1/MAP-65 and BMK-1/kinesin-5 act as a brake opposing the force generated by the spindle midzone. Conversely, we identify a novel role for two microtubule-growth and nucleation agents, Ran and CLASP, in the establishment of the centrosome-independent force during anaphase. Their involvement raises the interesting possibility that microtubule polymerization of midzone microtubules is continuously required to sustain chromosome segregation during mitosis.
DescripciónThis article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
Versión del editorhttp://dx.doi.org/10.1091/mbc.E14-12-1577
URIhttp://hdl.handle.net/10261/129463
DOI10.1091/mbc.E14-12-1577
Identificadoresdoi: 10.1091/mbc.E14-12-1577
e-issn: 1939-4586
issn: 1059-1524
Aparece en las colecciones: (CABD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Ran-andCLASP.pdf2,11 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.