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Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics

AuthorsSchulte-Zweckel, Janine; Rosi, Federica; Sreenu, Domalapally; Schröder, Hendrik; Niemeyer, Christof M.; Triola Guillem, Gemma
KeywordsAmino Acid Motifs
immobilized protein
fluorescent dye
Issue Date28-Oct-2014
PublisherRoyal Society of Chemistry
CitationChemical Communications
AbstractA novel technique for protein immobilization onto CrAsH-modified surfaces is presented. This approach enables an efficient, reversible and fluorogenic immobilization of proteins. Moreover, expressed proteins can also be directly immobilized from cellular lysates without prior purification. The immobilized proteins are suitable for protein-protein interaction studies and the fluorescence enhancement upon immobilization can be employed for the direct detection of the immobilized protein without the need for secondary detection methods.
Publisher version (URL)DOI: 10.1039/c4cc04120h
Appears in Collections:(IQAC) Artículos
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