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Título

The conserved transmembrane proteoglycan Perdido/Kon-tiki is essential for myofibrillogenesis and sarcomeric structure in Drosophila

AutorPérez-Moreno, Juan J. ; Bischoff, Marcus; Martín-Bermudo, María D. ; Estrada, Beatriz
Palabras clavePerdido
Myogenesis
Muscle
Myofibril
Sarcomere
Kon-tiki
Chondroitin sulfate proteoglycan
NG2
AN2
MCSP
CSPG4
Integrin
Fecha de publicación2014
EditorCompany of Biologists
CitaciónJournal of Cell Science 127(14): 3162-3173 (2014)
ResumenMuscle differentiation requires the assembly of high-order structures called myofibrils, composed of sarcomeres. Even though themolecular organization of sarcomeres is well known, the mechanisms underlying myofibrillogenesis are poorly understood. It has been proposed that integrin-dependent adhesion nucleates myofibrils at the periphery of the muscle cell to sustain sarcomere assembly. Here, we report a role for the gene perdido (perd, also known as kon-tiki, a transmembrane chondroitin proteoglycan) in myofibrillogenesis. Expression of perd RNAi in muscles, prior to adult myogenesis, can induce misorientation and detachment of Drosophila adult abdominal muscles. In comparison to controls, perd-depleted muscles contain fewer myofibrils, which are localized at the cell periphery. These myofibrils are detached from each other and display a defective sarcomeric structure. Our results demonstrate that the extracellular matrix receptor Perd has a specific role in the assembly of myofibrils and in sarcomeric organization. We suggest that Perd acts downstream or in parallel to integrins to enable the connection of nascent myofibrils to the Z-bands. Our work identifies the Drosophila adult abdominalmuscles as amodel to investigate in vivo the mechanisms behind myofibrillogenesis.
DescripciónThis is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Versión del editorhttp://dx.doi.org/10.1242/jcs.150425
URIhttp://hdl.handle.net/10261/129446
DOI10.1242/jcs.150425
Identificadoresdoi: 10.1242/jcs.150425
e-issn: 1477-9137
issn: 0021-9533
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