Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/129329
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Feedback regulation of SIN by Etd1 and Rho1 in fission yeast |
Autor: | Alcaide, María CSIC; Lahoz, Aurelia; Daga, Rafael R. CSIC ORCID; Jiménez-Martínez, Juan CSIC ORCID | Fecha de publicación: | 2014 | Editor: | Genetics Society of America | Citación: | Genetics 196(2): 455-470 (2014) | Resumen: | In fission yeast, the septation initiation network (SIN) is thought to promote cytokinesis by downstream activation of Rho1, a conserved GTPase that controls cell growth and division. Here we show that Etd1 and PP2A-Pab1, antagonistic regulators of SIN, are Rho1 regulators. Our genetic and biochemical studies indicate that a C-terminal region of Etd1 may activate Rho1 by directly binding it, whereas an N-terminal domain confers its ability to localize at the growing tips and the division site where Rho1 functions. In opposition to Etd1, our results indicate that PP2A-Pab1 inhibits Rho1. The SIN cascade is upstream-regulated by the Spg1 GTPase. In the absence of Etd1, activity of Spg1 drops down prematurely, thereby inactivating SIN. Interestingly, we find that ectopic activation of Rho1 restores Spg1 activity in Etd1-depleted cells. By using a cytokinesis block strategy, we show that Rho1 is essential to feedback-activate Spg1 during actomyosin ring constriction. Therefore, activation of Spg1 by Rho1, which in turn is regulated by Etd1, uncovers a novel feedback loop mechanism that ensures SIN activity while cytokinesis is progressing. | URI: | http://hdl.handle.net/10261/129329 | DOI: | 10.1534/genetics.113.155218 | Identificadores: | doi: 10.1534/genetics.113.155218 issn: 0016-6731 e-issn: 1943-2631 |
Aparece en las colecciones: | (CABD) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
16
checked on 07-abr-2024
SCOPUSTM
Citations
21
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
20
checked on 28-feb-2024
Page view(s)
193
checked on 24-abr-2024
Download(s)
90
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.